engleski

Resveratrol Inhibits Ovary Cells Proliferation Induced by Low Doses of Polychlorinated Biphenyls

Toxic potential of PCBs is linked to various deleterious effects on human health and, in 2016 IARC has upgraded the classification of PCBs to Group 1 "Carcinogenic to humans". Increased ROS levels by PCBs are able to promote tumor growth and malignant progression. This study was focused on low doses effects of PCB 77 congener at ovarian cellular level with the intention of reducing PCB induced proliferation by plant polyphenol-resveratrol. Resveratrol was selected because of protective effects on reproductive tissues and antioxidative activity. Initialy, ovary cells (CHO-K1 cell line) were exposed sole to resveratrol in order to determine non-toxic doses to further experiments. Dose dependent cell growth inhibition after resveratrol exposure (2.5-150 μM) was confirmed with Trypan Blue method with significant effects in concentrations ≥ 5 μM (p<0.025-p<0.001). Neutral Red method, unequal to results obtained with Trypan Blue method, showed statistically significant cell growth inhibition in doses above 50 μM (p<0.001), while lower doses have shown beneficial effects on cell viability. Cytofluorimetric analysis confirmed elevated late apoptotic/death cells fraction for resveratrol ≥50 μM and therefore concentrations lower than 50 μM were further used in experiments. Cells were pre-treated with resveratrol, then treated with 1 μM PCB 77 (most effective dose in stimulation of cell proliferation) and after 24h cell viability was determined. Presence of resveratrol efficiently suppressed PCB elevated cellular proliferation–statistically significant in doses 15-50 μM (p<0.05-p<0.01) confirmed with Trypan Blue method. These findings suggest that resveratrol alters cellular progression and may be effective in suppression of malignant progression.

PCB 77 ; protection ; plant polyphenol ; cell viability ; cance

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