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Pregled bibliografske jedinice broj: 954094

Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex


Župan, Gordana; Rajič Bumber, Jelena; Pilipović, Kristina; Gržeta, Nika; Župan, Željko; Križ, Jasna
Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex // 8th Annual Traumatic Brain Injury Conference
Washington, DC, SAD, 2018. (poster, podatak o recenziji nije dostupan, neobjavljeni rad, znanstveni)


CROSBI ID: 954094 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex

Autori
Župan, Gordana ; Rajič Bumber, Jelena ; Pilipović, Kristina ; Gržeta, Nika ; Župan, Željko ; Križ, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
8th Annual Traumatic Brain Injury Conference

Mjesto i datum
Washington, DC, SAD, 16-17.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
mouse ; repetitive traumatic brain injury ; transactivation response DNA-binding protein 43

Sažetak
Repetitive mild traumatic brain injury (rmTBI) is an entity associated with multiple concussions in athletes, and it is also of particular concern among military personnel. Transactivation response DNA-binding protein 43 (TDP-43) is a ubiquitous, highly conserved protein which is under physiological conditions predominantly positioned in the cell nuclei. In pathological conditions, TDP-43 translocates to the cytoplasm where it can become hyperphosphorylated, polyubiquitinated and cleaved and consequently forms cytoplasmic inclusions characteristically found in several neurodegenerative diseases such as amyotrophic lateral sclerosis, frontotemporal dementia and Alzheimer’s disease. Purpose of this preliminary study was to determine if repeated concussions have an effect on the expression of TDP-43 including its subcellular position, cleavage and phosphorylation in mice frontal cortex during the first week after the last brain trauma. Adult male animals were subjected to the rmTBI protocol for five consecutive days. They received head impacts twice daily, with 6 h intervals, according to the weight drop method (Kane et al., 2012). Control, sham-injured animals were only lightly anesthetized and went through the same procedure without receiving head impacts. Animals were sacrificed 1, 3 or 7 days after the last trauma or sham procedure and their frontal cortices were collected for the protein expression analyses. In the cortices of the injured mice, increased cytosolic TDP-43 expression accompanied with the higher cytosolic content of 35 kDa cleaved form of this protein were detected on the first day after the last brain trauma. Furthermore, in traumatized mice, increased TDP-43 phosphorylation was also found. Preliminary results of this study showed that rmTBI causes TDP-43 cytosol overexpression, its cleavage and phosphorylation in the mouse frontal cortex within the first week after the last head impact. These results confirm the suggestion that above mentioned posttraumatic TDP-43 transformation processes could be involved in the pathogenesis of different brain disorders linked with repeated concussions. This research was supported by the Croatian Science Foundation grant IP-2016-06-4602 to G.Ž.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-4602 - Blaga repetitivna traumatska ozljeda mozga: model za istraživanje neuropatologije i neuroupale posredovane s TDP-43 (TDP-43repTBI) (Župan, Gordana, HRZZ - 2016-06) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka


Citiraj ovu publikaciju:

Župan, Gordana; Rajič Bumber, Jelena; Pilipović, Kristina; Gržeta, Nika; Župan, Željko; Križ, Jasna
Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex // 8th Annual Traumatic Brain Injury Conference
Washington, DC, SAD, 2018. (poster, podatak o recenziji nije dostupan, neobjavljeni rad, znanstveni)
Župan, G., Rajič Bumber, J., Pilipović, K., Gržeta, N., Župan, Ž. & Križ, J. (2018) Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex. U: 8th Annual Traumatic Brain Injury Conference.
@article{article, author = {\v{Z}upan, Gordana and Raji\v{c} Bumber, Jelena and Pilipovi\'{c}, Kristina and Gr\v{z}eta, Nika and \v{Z}upan, \v{Z}eljko and Kri\v{z}, Jasna}, year = {2018}, keywords = {mouse, repetitive traumatic brain injury, transactivation response DNA-binding protein 43}, title = {Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex}, keyword = {mouse, repetitive traumatic brain injury, transactivation response DNA-binding protein 43}, publisherplace = {Washington, DC, SAD} }
@article{article, author = {\v{Z}upan, Gordana and Raji\v{c} Bumber, Jelena and Pilipovi\'{c}, Kristina and Gr\v{z}eta, Nika and \v{Z}upan, \v{Z}eljko and Kri\v{z}, Jasna}, year = {2018}, keywords = {mouse, repetitive traumatic brain injury, transactivation response DNA-binding protein 43}, title = {Repetitive mild traumatic brain injury increases cytosolic expression, cleavage and phosphorylation of TDP-43 in the mouse frontal cortex}, keyword = {mouse, repetitive traumatic brain injury, transactivation response DNA-binding protein 43}, publisherplace = {Washington, DC, SAD} }




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