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Antibacterial assesment of aroylhydrazone derivatives in vitro

Aroylhydrazones have attracted considerable attention for their wide range of biological activities, such as antibacterial, anti-fungal, antitumor and anti-inflammatory properties.1, 2, 3 Additionally, hydrazones as chelating agents were investigated as potential drugs for treatment of iron-overload associated diseases.4 In the present work, a group of 13 derivatives was studied for antibacterial activity, as a part of our investigations on aroylhydrazones. The Staphylococcus aureus ATCC 6538 and E. coli ATCC 10536 was used as a model strains. Modified serial and twofold microdilution method according to the EUCAST 5.1 recommendations was used to determine OD540 after incubation. MIC (as IC90) and IC50 was calculated as %viability/logconc ratio by non- linear regression and Gompertz equitation using GraphPad Prism 7.0. The tests were performed in triplicated and MIC (IC90) and IC50 presented as mean. Compounds 11 and 12 showed antibacterial activity against S. aureus and E. coli strains with MICs between 33.02 and 104.9 μg/mL while 6 and 8 showed activity only against S. aureus with MICs range from 129.7 to 270.3 μg/mL. Compounds 2 and 10 were active only against E. coli with MIC values 55.58 and 50.47 μg/mL, respectively. IC50 values reveals that compounds 6, 8 and 11 exhibited strong bactericidal activity against S. aureus strain represented as IC50.

Aroylhydrazones ; Antibacterial activity

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