engleski

Difficulties in diagnosis and new insights in risk factors of canid alphaherpesvirus -1 infection

Canid herpesvirus-1 (CaHV-1) is enzootic pathogen of dogs often related to reproductive disorders. Unfortunately, substantial data about risk factors influencing seroconversion and spreading of the infection are scarce. In period from 2014 to 2017 a total of 128 breeding bitches, with no history of vaccination, were randomly sampled from 24 different breeding kennels in Croatia. From each animal multiple sera samples, vaginal and nasal swabs were collected in different stages of reproductive cycle. CaHV-1 antibody titre was determined using virus neutralisation assay and possible viral excretion using PCR method described earlier. Investigated risk factors which could affect on level of CaHV-1 antibody titres were: age, estrous cycle, number of mating, kennel size, attendance at dog shows, attendance at hunt and hygiene. Statistical analyses were performed using Statistica v.13 (TIBCO Software Inc, 2017). Data (antibody titres) were assessed for significance using ANOVA.

Canid alphaherpesvirus-1, diagnosis, risk factors

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