Napredna pretraga

Pregled bibliografske jedinice broj: 952693

Novel approach in developing Saccharomyces cerevisiae hybrid bioethanol producers by mating of natural isolates having desirable traits


Žunar, Bojan; Štafa, Anamarija; Zandona, Antonio; Svetec Miklenić, Marina; Šantek, Božidar; Svetec, Ivan-Krešimir
Novel approach in developing Saccharomyces cerevisiae hybrid bioethanol producers by mating of natural isolates having desirable traits // Journal of Biotechnology, Volume 280, Supplement / Keskin, Burak (ur.).
Gangtok, India: Thomson Digital, 2018. str. S89-S89 doi:10.1016/j.jbiotec.2018.06.294 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Novel approach in developing Saccharomyces cerevisiae hybrid bioethanol producers by mating of natural isolates having desirable traits

Autori
Žunar, Bojan ; Štafa, Anamarija ; Zandona, Antonio ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan-Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Biotechnology, Volume 280, Supplement / Keskin, Burak - Gangtok, India : Thomson Digital, 2018, S89-S89

Skup
European Biotechnology Congress 2018

Mjesto i datum
Atena, Grčka, 26-28.04.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Saccharomyces cerevisiae, hibridi, bioetanol
(Sacchromyces cerevisae, hybrids, bioethanol)

Sažetak
Bioethanol production from lignocellulosic hydrolysates requires a producer strain that tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous intraspecies hybrid diploids of Saccharomyces cerevisiae by crossing two natural S. cerevisiae isolates, a good ethanol producer found in wine and a strain resistant to inhibitors found in lignocellulosic hydrolysates. Compared to parental strains, hybrids grew faster in the absence, as well as in the presence of acetic and levulinic acid and 2-furaldehyde, inhibitors frequently found in lignocellulosic hydrolysates. It was shown that the overexpression of YAP1 gene, involved in a stress response, further increased the survival. Furthermore, hybrids displayed different fermentative potential in a CO2 production test suggesting genetic variability that could be used for further selection of desirable traits. Therefore, our results suggest that the construction of intraspecies hybrids coupled with use of genetic engineering techniques is a promising approach for an improvement or development of new biotechnologically relevant strains of S. cerevisiae.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-9158 - Održiva proizvodnja bioetanola i biokemikalija iz otpadnih poljoprivrednih lignoceluloznih sirovina (Božidar Šantek, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI


Citati