engleski

True to type confirmation of twenty unknown grapevine accessions at grape germplasm collection in Split, Croatia

The grape germplasm collection in Institute for Adriatic Crops and Karst Reclamation (IAC, HRV048) Split is one of core collections in Croatia. In line with the wider endeavours of long-term conservation and utilization of plant genetic resources in Europe, it places special importance on maintaining genetic diversity of predominantly native Dalmatian varieties. Although IAC collection is already being largely genotyped, 20 accessions maintained in the field collection had unknown or unconfident cultivar status and needed verification of trueness to type. These unknown accessions were accessed from different academic and non- academic sources. Morphological validation was done by standard OIV descriptors encompassing shoot, leaf and cluster characteristics. A standard set of 9 simple sequence repeats (SSR) were amplified and genetic diversity indices were calculated. The allelic data of 20 unknown genotypes were compared with reference SSR profiles from the European database Vitis International Variety Catalogue (VIVC, http://www.vivc.de/) and grape germplasm collection of the United Kingdom. Three accessions proved to be mislabelled and identified as known European grapevine cultivars (Blaufraenkisch, Chardonnay, Merlot), two unknown accessions showed identical genotypes matching Chasselas blanc and Chasellas rose, while 12 unknown genotypes confirmed their declared status. Finally, 3 unknown genotypes of interspecific crosses (Ljana, Orginal, Nero) were not found in VIVC database nor in published literature revealing novel SSR profiles to our knowledge. This study presents necessary information to establish cultivar status (true to type) of 20 unknown accessions from IAC Split grape collection providing clear identity of accessions that is essential for further collection management and achieving maximum genetic diversity in a limited collection capacity.

SSR markers, ampelography, grapevine genetic diversity

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