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Clinically significant influence of haemolysis on 25 biochemistry parameters (CROSBI ID 665008)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Perović, Antonija ; Dolčić, Maja Clinically significant influence of haemolysis on 25 biochemistry parameters // Biochemia medica. 2018. str. 95-96

Podaci o odgovornosti

Perović, Antonija ; Dolčić, Maja

engleski

Clinically significant influence of haemolysis on 25 biochemistry parameters

Introduction: Manufacturers’ recommendations on hemolysis influence on tests parameters are often scarce, non-harmonized and therefore should be evaluated prior to implementation in routine work. The aim of this study was to investigate influence of hemolysis on four different hemolysis levels for 25 biochemistry parameters and to evaluate the clinical significance of hemolysis in order to prevent unnecessary sample rejection. Materials and Methods: A total of 17 venous blood samples collected in gel-separator tubes were included in the study. The samples were immediately spiked with high concentrations of tested parameters and a 1 mL aliquot was taken for hemolysate preparation by rapid freeze-thaw method. The non- hemolyzed serum was divided into five aliquots. Four hemolysis levels were prepared by adding hemolyzed serum. Hemolysis index (HI) and hemoglobin concentration in prepared samples were classified as following: (+)=0.5-0.99 g/L, (++)=1- 1.99 g/L, (+++)=2-2.99 g/L, (++++)=3- 4.99 g/L. The following parameters were determined on Beckman Coulter AU680 analyzer: IH, potassium, sodium, chloride, calcium, magnesium, inorganic phosphates, iron, glucose, total and conjugated bilirubin, urea, creatinine, uric acid, alkaline phosphatase (ALP), gamma- glutamyltransferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT) creatine kinase (CK), CK- MB, lactate dehydrogenase (LD), alpha- amylase, cholinesterase, C-reactive protein (CRP), total protein, albumin. The percentage differences between non- hemolytic and hemolytic serum judged against desirable bias (DSB) derived from biological variation and reference change values (RCV) calculated according to the following formula: RCV=21/2×Z×(CVA2+CVI2)1/2, where Z=1, 96 ; CVA=calculated analytical imprecision ; CVI= intraindividual biological variation. The difference higher than RCV was considered clinically significant. Results: The differences exceeded DSB for: potassium, total and conjugated bilirubin, AST, CK-MB, LD at IH= (+) ; sodium, magnesium, inorganic phosphates, GGT, total protein at IH=(++) ; calcium, CK, alpha-amylase, albumin at IH= (+++). The differences exceeded RCV for: LD at IH=(+) ; CK-MB at IH=(++) ; potassium, AST at IH=(+++). Conclusion: Hemolysis influence is clinically significant for LD at IH=(+), CK-MB at IH=(++), potassium, AST at IH=(+++).

Hemolysis ; Interference ; Laboratory testing ; Preanalytical errors ; Reference change value

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Podaci o prilogu

95-96.

2018.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Biochemia medica

1330-0962

1846-7482

Podaci o skupu

9. kongres hrvatskog društva za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM)

poster

09.05.2018-12.05.2018

Zagreb, Hrvatska

Povezanost rada

nije evidentirano

Indeksiranost