engleski

Veronica spicata L. - HPLC Profile of Phenolic Compounds in Hydrolates

Genus Veronica L. (Plantaginaceae) is divided into 13 subgenuses and 270 species, of whom 40 are widespread in Croatia [1]. Samples of the investigated Veronica spicata L. were collected in two localities, mountains Dinara and Velebit. They are cosmopolitan and ecologically diverse species spread on a variety of habitats from aquatic, marshy and forest to rock, rock cracks, fields and ruderal habitats [2]. Some of the species from the genus Veronica have been used in traditional medicine. Literature reviews showed that the most investigated secondary metabolites for Veronica genus include iridoid glucosides, phenylethanoids and flavonoid glycosides [3], so this is the first study of the chemical composition of the essential waters - hydrolates from this species. Hydrolates are aqueous products of hydrodistillation from aerial parts of plant. Aerial parts of the two samples of V. spicata were air dried and subjected to hydrodistillation in Clevenger type apparatus. 2mL of pentane and 40 mL of water was added to the inner part of the graduated tube at each distillation. At the end of the process of distillation the organic and water layer were separated and refrigerated until the analyses [4]. The phenolic compounds from hydrolates have been analyzed by Perkin Elmer HPLC system (Waltham, Massachusetts, USA) using the C18 column (Ultra-Aqueous C-18, 250 x 4.6 mm, 5 Å) (Restek, USA). Components 3, 4-dihydroxybenzoic acid and vanillin were identified, with the following values. Component in the Sample 1 contained 3.47 mg/L of 3, 4-dihydroxybenzoic acid and the Sample 2 contained 6.12 mg/L. Component vanillin in the Sample 1 is represented with 0.12 mg/L and in the Sample 2 with 0.76 mg/L. Our previous studies have included research of volatile components in the composition of the essential oil of V. spicata analyzed by GC/MS [4]. Du and Jin found vanillic acid in the species Veronica peregrine [5]. The present study gives additional knowledge about volatile compounds in the hydrolates of the genus Veronica.

Veronica ; HPLC ; 3, 4-dihydroxybenzoic acid ; vanillin

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