Ion-exchange chromatography using CIM QA as a final polishing step in horse F(ab’)2-based immunotherapeutics preparation (CROSBI ID 664966)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kurtović, Tihana ; Brgles, Marija ; Halassy, Beata
engleski
Ion-exchange chromatography using CIM QA as a final polishing step in horse F(ab’)2-based immunotherapeutics preparation
Horse F(ab')2-based biomedicines are the only effective and specific therapy against snakebite. In general, F(ab')2 are produced by pepsin hydrolysis of immunoglobulin G molecules since Fc removal reduces their side-effects-inducing potential, thus generating drug of improved safety more suitable for use in human. The final product should be free of residual contaminants from fractionation process, thus, elimination of pepsin - the main stability-compromising agent, should be guaranteed. This study aimed to investigate the possibility of employing ion-exchange chromatography using CIM QA disk as a method of choice for the final polishing - removal of any processing by-product traces, primarily pepsin. Namely, development of our F(ab’)2 purification processing strategy has been guided by the imperative of constantly keeping active compound (IgG or F(ab’)2) in solution, since possible degradation/aggregation due to precipitation or binding to chromatographic support might lead to reduced efficacy and/or stability of the final product. We have successfully developed chromatographic conditions enabling complete binding of pepsin to CIM QA disk, while leaving F(ab’)2 fragments in the flow through fraction. The yield of the final polishing by the novel method is near 100%. Described chromatographic procedure was also shown to be applicable for further purification of commercial pepsin preparation that is often contaminated with non-enzymatically active material.
antivenom ; immunoglobulin purification ; CIM QA chromatography
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Podaci o prilogu
45-45.
2018.
objavljeno
Podaci o matičnoj publikaciji
Monolith Summer School and Symposium
Podaci o skupu
Monolith Summer School and Symposium
poster
15.06.2018-20.06.2018
Portorož, Slovenija