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Pregled bibliografske jedinice broj: 951901

Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes


Vukojević, Katarina; Raguž, Fila; Saraga, Marijan; Filipović, Natalija; Bočina, Ivana; Kero, Darko; Glavina Durdov, Merica; Martinović, Vlatka; Saraga-Babić, Mirna
Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes // Acta histochemica, 120 (2018), 8; 748-756 doi:10.1016/j.acthis.2018.08.015 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 951901 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes

Autori
Vukojević, Katarina ; Raguž, Fila ; Saraga, Marijan ; Filipović, Natalija ; Bočina, Ivana ; Kero, Darko ; Glavina Durdov, Merica ; Martinović, Vlatka ; Saraga-Babić, Mirna

Izvornik
Acta histochemica (0065-1281) 120 (2018), 8; 748-756

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
kidney development ; podocytes ; nephrin ; primary cilia ; nestin ; CNF

Sažetak
Background: Podocytes are postmitotic, highly specialized cells which maintain the glomerular filtration barrier (GFB). Their injury is characterized by foot processes effacement and change in protein expression leading to proteinuria and end-stage kidney disease. Methods: Our study focuses on the morphological and immunohistochemical changes of human podocytes during normal development and postnatal period, compared to congenital nephrotic syndrome of the Finnish type (CNF). Kidney tissues taken from 17 human conceptuses 8th-38th weeks old, two healthy and three CNF kidneys were embedded in paraffin for immunohistochemical or double immunofluorescence methods, or were embedded in resin for electron microscopy. Paraffin sections were stained with markers for proliferation (Ki-67), proteins nephrin and nestin, and alpha-tubulin. Quantification of positive cells were performed using Mann Whitney and Kruskal-Wallis test. Results: Tissue analysis showed that proliferation of podocytes gradually decreased during development and disappeared in postnatal period. Decrease in number of ciliated glomerular cells and visceral podocytes (from 47% to 3%), and parietal epithelial cells (from 32% to 7%) characterized normal development. Nestin and nephrin co-expressed in developing podocytes in different cellular compartments. During development, nephrin expression increased (from 17% to 75%) and postnatally changed its pattern, while nestin positive glomerular cells decreased from 98% to 40%. CNF glomeruli displayed increased number of immature ciliated podocytes (6%) and parietal epithelial cells (9%). Conclusion: Changes in cytoplasmic alpha-tubulin expression and reduced nephrin expression (20%) indicating association of incomplete podocyte maturation with failure of GFB function and appearance of prenatal proteinuria in CNF patients.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
MZOS-216-2160528-0507 - Genski izražaj u ranom razvoju čovjeka (Saraga-Babić, Mirna, MZOS ) ( POIROT)
HRZZ-IP-2016-06-2575 - Karakterizacija kandidat gena za kongenitalne anomalije bubrega i urotrakta (CAKUT) tijekom razvoja u miša i čovjeka (CAKUT) (Vukojević, Katarina, HRZZ - 2016-06) ( POIROT)

Ustanove:
KBC Split,
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split

Poveznice na cjeloviti tekst rada:

doi ur.booksc.eu

Citiraj ovu publikaciju:

Vukojević, Katarina; Raguž, Fila; Saraga, Marijan; Filipović, Natalija; Bočina, Ivana; Kero, Darko; Glavina Durdov, Merica; Martinović, Vlatka; Saraga-Babić, Mirna
Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes // Acta histochemica, 120 (2018), 8; 748-756 doi:10.1016/j.acthis.2018.08.015 (međunarodna recenzija, članak, znanstveni)
Vukojević, K., Raguž, F., Saraga, M., Filipović, N., Bočina, I., Kero, D., Glavina Durdov, M., Martinović, V. & Saraga-Babić, M. (2018) Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes. Acta histochemica, 120 (8), 748-756 doi:10.1016/j.acthis.2018.08.015.
@article{article, author = {Vukojevi\'{c}, Katarina and Ragu\v{z}, Fila and Saraga, Marijan and Filipovi\'{c}, Natalija and Bo\v{c}ina, Ivana and Kero, Darko and Glavina Durdov, Merica and Martinovi\'{c}, Vlatka and Saraga-Babi\'{c}, Mirna}, year = {2018}, pages = {748-756}, DOI = {10.1016/j.acthis.2018.08.015}, keywords = {kidney development, podocytes, nephrin, primary cilia, nestin, CNF}, journal = {Acta histochemica}, doi = {10.1016/j.acthis.2018.08.015}, volume = {120}, number = {8}, issn = {0065-1281}, title = {Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes}, keyword = {kidney development, podocytes, nephrin, primary cilia, nestin, CNF} }
@article{article, author = {Vukojevi\'{c}, Katarina and Ragu\v{z}, Fila and Saraga, Marijan and Filipovi\'{c}, Natalija and Bo\v{c}ina, Ivana and Kero, Darko and Glavina Durdov, Merica and Martinovi\'{c}, Vlatka and Saraga-Babi\'{c}, Mirna}, year = {2018}, pages = {748-756}, DOI = {10.1016/j.acthis.2018.08.015}, keywords = {kidney development, podocytes, nephrin, primary cilia, nestin, CNF}, journal = {Acta histochemica}, doi = {10.1016/j.acthis.2018.08.015}, volume = {120}, number = {8}, issn = {0065-1281}, title = {Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes}, keyword = {kidney development, podocytes, nephrin, primary cilia, nestin, CNF} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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