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Strong impact of chloro substituent on TOTO and YOYO ds-DNA/RNA sensing


Rožman, Andrea; Crnolatac, Ivo; Deligeorgiev, Todor; Piantanida, Ivo
Strong impact of chloro substituent on TOTO and YOYO ds-DNA/RNA sensing // Journal of luminescence, 205 (2019), 87-96 doi:10.1016/j.jlumin.2018.08.074 (međunarodna recenzija, članak, znanstveni)


Naslov
Strong impact of chloro substituent on TOTO and YOYO ds-DNA/RNA sensing

Autori
Rožman, Andrea ; Crnolatac, Ivo ; Deligeorgiev, Todor ; Piantanida, Ivo

Izvornik
Journal of luminescence (0022-2313) 205 (2019); 87-96

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA and RNA recognition ; fluorescent markers ; circular dichroism ; target-aggregating dyes

Sažetak
Introduction of chloro-substituent at cyanine chromophore of well-known DNA stains TOTO and YOYO resulted in a significant difference of DNA and RNA recognition in respect to parent compounds (TOTO ; YOYO), as well as in respect to corresponding monomers (Cl-TO and Cl-YO). Studied novel compounds showed strong, submicromolar affinity to all ds-polynucleotides, with minor differences in binding constant between various DNA and RNA. The Cl-YOYO revealed a specific fluorimetric recognition of ds-RNA in respect to ds-DNA by the fluorimetric response at 570 nm, which was attributed to particular aggregation property of dye, not present in corresponding Cl-TOTO analogue. The Cl-TOTO, unlike YO-analogue, showed ICD band at 520 nm only upon binding to alternating DNA sequences (both, GC-GC and AT-AT), which was not observed for homo-DNA and homo-RNA. Such recognition pattern was attributed to structural differences between polynucleotides, whereby only alternating-DNAs have a typical B-helical structure in which Cl-TOTO molecule could self-fold to give particular ICD band at 520 nm. Thus, both Cl-analogues show intriguing novel recognition properties in respect to parent TOTO and YOYO, which combined with negligible cytotoxicity offer potential for development of novel dyes for biochemical and biomedical applications.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA / RNA sekundarne strukture molekularnim kemijskim senzorima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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