Effect of melatonin and resveratrol on the expression of aquaporin 1 and 2 in scenecent male and female rats (CROSBI ID 664130)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Karaica, Dean ; Breljak, Davorka ; Vrhovac Madunić, Ivana ; Micek, Vedran ; Ljubojević, Marija ; Gerić, Marko ; Gajski, Goran ; Rašić, Dubravka ; Peraica, Maja ; Orct, Tatjana ; Jurasović, Jasna ; Novak Jovanović, Ivana ; Nanić, Lucia ; Rubelj, Ivica ; Sabolić, Ivan
engleski
Effect of melatonin and resveratrol on the expression of aquaporin 1 and 2 in scenecent male and female rats
Water is the most abundant molecule in living cells. Aquaporin protein channels (AQPs) facilitate the passive movement of water across cell membranes. In mammalian kidneys, AQP1 and AQP2 facilitate water reabsorption from primary urine across the apical membrane of proximal and distal nephron tubules. Studies indicate that renal functional/structural changes in humans and experimental animals develop with age. Aging promotes fluid loss in tissues which was suggested to reflect changes in the expression/activity of AQPs. Here we investigated the potential age-ameliorating effects of two potential geroprotector substances, melatonin (endogenous hormone) and resveratrol (plant flavonoid), on AQP1 and AQP2 expressions in the rat model of experimental aging. During 21 months, rats of both sexes were given melatonin or resveratrol via drinking water (~1 mg/kg b.w./day), whereas control animals drank vehicle (0.01% ethanol in drinking water). The expression of both AQPs in the kidneys of control and treated animals was determined using immunofluorescence cytochemistry in tissue cryosections and Western blot analysis in isolated cell membranes. Results showed that melatonin and resveratrol treatment had no effect on renal AQP1 expression in both sexes. AQP2 expression in male and female kidneys of resveratrol-treated rats also did not change compared to controls. However, only in female rats, AQP2 expression was upregulated by melatonin in the inner stripe and papilla kidney regions. Therefore, in 24-month old rat kidneys, chronic oral intake of melatonin and resveratrol had no effect on AQP1, whereas melatonin exerted sex-dependent upregulation of AQP2. (Supported by Croatian Science Foundation project IP-2013-11-1481).
aging ; antioxidants ; aquaporins ; chronic exposure ; immunochemical analyses ; kidneys
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Podaci o prilogu
126-126.
2018.
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objavljeno
Podaci o matičnoj publikaciji
FEBS Open Bio
2211-5463
Podaci o skupu
43rd FEBS Congress ''Biochemistry Forever''
poster
07.07.2018-12.07.2018
Prag, Češka Republika
Povezanost rada
Temeljne medicinske znanosti