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Pregled bibliografske jedinice broj: 947745

Effect of melatonin and resveratrol on the expression of aquaporin 1 and 2 in scenecent male and female rats


Karaica, Dean; Breljak, Davorka; Vrhovac Madunić, Ivana; Micek, Vedran; Ljubojević, Marija; Gerić, Marko; Gajski, Goran; Rašić, Dubravka; Peraica, Maja; Orct, Tatjana et al.
Effect of melatonin and resveratrol on the expression of aquaporin 1 and 2 in scenecent male and female rats // Final programme
Prague: Czech Society for Biochemistry and Molecular Biology, 2018. str. 126-126 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Effect of melatonin and resveratrol on the expression of aquaporin 1 and 2 in scenecent male and female rats

Autori
Karaica, Dean ; Breljak, Davorka ; Vrhovac Madunić, Ivana ; Micek, Vedran ; Ljubojević, Marija ; Gerić, Marko ; Gajski, Goran ; Rašić, Dubravka ; Peraica, Maja ; Orct, Tatjana ; Jurasović, Jasna ; Novak Jovanović, Ivana ; Nanić, Lucia ; Rubelj, Ivica ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final programme / - Prague : Czech Society for Biochemistry and Molecular Biology, 2018, 126-126

Skup
The 43rd FEBS Congress

Mjesto i datum
Prag, Republika Češka, 07-12.07.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aging, antioxidants, aquaporins, chronic exposure, immunochemical analyses, kidneys

Sažetak
Water is the most abundant molecule in living cells. Aquaporin protein channels (AQPs) facilitate the passive movement of water across cell membranes. In mammalian kidneys, AQP1 and AQP2 facilitate water reabsorption from primary urine across the apical membrane of proximal and distal nephron tubules. Studies indicate that renal functional/structural changes in humans and experimental animals develop with age. Aging promotes fluid loss in tissues which was suggested to reflect changes in the expression/activity of AQPs. Here we investigated the potential age-ameliorating effects of two potential geroprotector substances, melatonin (endogenous hormone) and resveratrol (plant flavonoid), on AQP1 and AQP2 expressions in the rat model of experimental aging. During 21 months, rats of both sexes were given melatonin or resveratrol via drinking water (~1 mg/kg b.w./day), whereas control animals drank vehicle (0.01% ethanol in drinking water). The expression of both AQPs in the kidneys of control and treated animals was determined using immunofluorescence cytochemistry in tissue cryosections and Western blot analysis in isolated cell membranes. Results showed that melatonin and resveratrol treatment had no effect on renal AQP1 expression in both sexes. AQP2 expression in male and female kidneys of resveratrol-treated rats also did not change compared to controls. However, only in female rats, AQP2 expression was upregulated by melatonin in the inner stripe and papilla kidney regions. Therefore, in 24-month old rat kidneys, chronic oral intake of melatonin and resveratrol had no effect on AQP1, whereas melatonin exerted sex-dependent upregulation of AQP2. (Supported by Croatian Science Foundation project IP-2013-11-1481).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1481 - Starosno-ovisna ekspresija membranskih prijenosnika u štakora (Ivan Sabolić, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb