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Pregled bibliografske jedinice broj: 947631

Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity


Spidlova, Petra; Stojkova, Pavla; Dankova, Vera; Senitkova, Iva; Santic, Marina; Pinkas, Pinkas; Philimonenko, Vlada and Stulík, Jiri
Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity // Frontiers in Cellular and Infection Microbiology, 8 (2018), 111; 1-14 (međunarodna recenzija, članak, znanstveni)


Naslov
Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity

Autori
Spidlova, Petra ; Stojkova, Pavla ; Dankova, Vera ; Senitkova, Iva ; Santic, Marina ; Pinkas, Pinkas ; Philimonenko, Vlada and Stulík, Jiri

Izvornik
Frontiers in Cellular and Infection Microbiology (2235-2988) 8 (2018), 111; 1-14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Francisella, DacD, virulence, phagosomal escape, carboxypeptidase, penicillin binding proteins,

Sažetak
D-alanyl-D-alanine carboxypeptidase, product of dacD gene in Francisella, belongs to penicillin binding proteins (PBPs) and is involved in remodeling of newly synthetized peptidoglycan. In E. coli, PBPs are synthetized in various growth phases and they are able to substitute each other to a certain extent. The DacD protein was found to be accumulated in fraction enriched in membrane proteins from severely attenuated dsbA deletion mutant strain. It has been presumed that the DsbA is not a virulence factor by itself but that its substrates, whose correct folding and topology are dependent on the DsbA oxidoreductase and/or isomerase activities, are the primary virulence factors. Here we demonstrate that Francisella DacD is required for intracellular replication and virulence in mice. The dacD insertion mutant strain showed higher sensitivity to acidic pH, high temperature and high osmolarity when compared to the wild-type. Eventually, transmission electron microscopy revealed differences in mutant bacteria in both the size and defects in outer membrane underlying its SDS and serum sensitivity. Taken together these results suggest DacD plays an important role in Francisella pathogenicity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2016-06-9003 - Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije (Marina Šantić, )
UNIRI 811.10.1111

Ustanove
Medicinski fakultet, Rijeka

Autor s matičnim brojem:
Marina Šantić, (242464)

Citiraj ovu publikaciju

Spidlova, Petra; Stojkova, Pavla; Dankova, Vera; Senitkova, Iva; Santic, Marina; Pinkas, Pinkas; Philimonenko, Vlada and Stulík, Jiri
Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity // Frontiers in Cellular and Infection Microbiology, 8 (2018), 111; 1-14 (međunarodna recenzija, članak, znanstveni)
Spidlova, P., Stojkova, P., Dankova, V., Senitkova, I., Santic, M., Pinkas, P. & Philimonenko, Vlada and Stulík, Jiri (2018) Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity. Frontiers in Cellular and Infection Microbiology, 8 (111), 1-14.
@article{article, year = {2018}, pages = {1-14}, keywords = {Francisella, DacD, virulence, phagosomal escape, carboxypeptidase, penicillin binding proteins,}, journal = {Frontiers in Cellular and Infection Microbiology}, volume = {8}, number = {111}, issn = {2235-2988}, title = {Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity}, keyword = {Francisella, DacD, virulence, phagosomal escape, carboxypeptidase, penicillin binding proteins,} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE