engleski

Determination of aflatoxin M1 in milk using highperformance liquid chromatography-tandem mass spectrometry

Growth of mold on cereal grains may result in the formation of secondary metabolites – mycotoxins. [1] Mycotoxins may occur during any stage of production and transformation into a food product. They can be produced in plants infected in the field, during harvesting, storage and transport, technological transformation and food preparation. [2] Aflatoxins are one of the most significant and most serious causes of mycotoxicosis in humans and animals. [1] They are primarily produced by two species of Aspergillus fungus (A. flavus and A. parasiticus). A. flavus produces B aflatoxins and A. parasiticus produces B and G aflatoxins. [3] Aflatoxins are extremely toxic, mutagenic, teratogenic and carcinogenic compounds. Aflatoxin B1 (AFB1) is the most carcinogen aflatoxin. [2] Aflatoxin M1 (AFM1) is a hydroxylated product of AFB1 and it is found in animal and human breast milk. [1] AFM1 is a relatively stable compound in raw and processed milk. Since it can't be eliminated by pasteurization it represents a serious health concern. [2] In this study the method for determination AFM1 in milk samples has been validated according to the Commission Decision 2002/657/EC. The samples were defatted and passed through the immunoaffinity columns (IAC) VICAM Afla M1TM HPLC. Acetonitrile was used for elution. After evaporation the samples were reconstituted in ultrapure water and acetonitrile, and analyzed by the HPLC-MS/MS.

mycotoxins ; aflatoxin M1 ; HPLC-MS/MS

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