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Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry


Peharec Štefanić, Petra; Cindrić, Mario; Balen, Biljana
Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry // Plant Cell Culture Protocols, Methods in Molecular Biology, vol. 1815 / Loyola-Vargas, Víctor M ; Ochoa-Alejo, Neftalí (ur.).
New York: Springer Protocols, Humana Press, 2018. str. 351-370 doi:10.1007/978-1-4939-8594-4_25


Naslov
Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry

Autori
Peharec Štefanić, Petra ; Cindrić, Mario ; Balen, Biljana

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Plant Cell Culture Protocols, Methods in Molecular Biology, vol. 1815

Urednik/ci
Loyola-Vargas, Víctor M ; Ochoa-Alejo, Neftalí

Izdavač
Springer Protocols, Humana Press

Grad
New York

Godina
2018

Raspon stranica
351-370

ISBN
978-1-4939-8594-4

ISSN
1064-3745

Ključne riječi
De novo sequencing ; Isoelectric focusing ; Phenol extraction ; SDS-PAGE ; Two-dimensional electrophoresis ; MALDI-MS/MS

Sažetak
Separation of plant proteins by means of electrophoretic techniques is quite challenging since different compounds typical for plant cells can interfere and/or reduce the effectiveness of the protein isolation. This is particularly problematic for two-dimensional electrophoresis (2-DE). Therefore, it is important to optimize protein extraction and to establish a robust protocol for 2-DE and downstream processing, primarily mass spectrometry (MS) analysis. Here we give a detailed protocol for protein extraction using phenol method, 2-DE, and MALDI-MS analysis.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Peharec Štefanić, Petra; Cindrić, Mario; Balen, Biljana
Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry // Plant Cell Culture Protocols, Methods in Molecular Biology, vol. 1815 / Loyola-Vargas, Víctor M ; Ochoa-Alejo, Neftalí (ur.).
New York: Springer Protocols, Humana Press, 2018. str. 351-370 doi:10.1007/978-1-4939-8594-4_25
Peharec Štefanić, P., Cindrić, M. & Balen, B. (2018) Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry. U: Loyola-Vargas, V. & Ochoa-Alejo, N. (ur.) Plant Cell Culture Protocols, Methods in Molecular Biology, vol. 1815. New York, Springer Protocols, Humana Press, str. 351-370 doi:10.1007/978-1-4939-8594-4_25.
@inbook{inbook, year = {2018}, pages = {351-370}, DOI = {10.1007/978-1-4939-8594-4\_25}, keywords = {De novo sequencing, Isoelectric focusing, Phenol extraction, SDS-PAGE, Two-dimensional electrophoresis, MALDI-MS/MS}, doi = {10.1007/978-1-4939-8594-4\_25}, isbn = {978-1-4939-8594-4}, issn = {1064-3745}, title = {Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry}, keyword = {De novo sequencing, Isoelectric focusing, Phenol extraction, SDS-PAGE, Two-dimensional electrophoresis, MALDI-MS/MS}, publisher = {Springer Protocols, Humana Press}, publisherplace = {New York} }

Časopis indeksira:


  • Scopus


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