Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde (CROSBI ID 663939)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Česnik, Morana ; Sudar, Martina ; Roldan, Raquel ; Hernandez, Karel ; Clapés, Pere ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana
engleski
Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde
Carbon-carbon bond formation between propanal and formaldehyde in aldol addition catalysed by newly developed D-fructose-6-phosphate aldolase variant was investigated in this work. The reaction product is 3-hydroxy-2-methylpropanal (Roche aldehyde) widely used as intermediate in synthetic chemistry. The reaction kinetics was determined and the mathematical model was developed for process optimization. Optimization goals included finding the best conditions and the best reactor mode to obtain maximum product concentration and yield. It was found that the kinetics of the aldol addition can be described by double substrate Michaelis-Menten kinetics with included substrate inhibition by formaldehyde and propanal and noncompetitive inhibition by methanol used as formaldehyde stabilizer. Self-addition of propanal was described by double substrate Michaelis-Menten kinetics and included in the model. It was found that enzyme operational stability decay is co-dependent on the initial formaldehyde concentration. Thus, the choice of the initial conditions is crucial for the successful process set-up. Developed mathematical model described the experimental data very well. At the calculated optimal process conditions in fed-batch as the best reactor choice, the product concentration after 5.5 hours was 814 mmol dm-3 (72 g L–1), product yield was 88.5% and volume productivity was 313.7 g L–1 d–1.
aldol addition, aldolase, mathematical modelling
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Podaci o prilogu
137-137.
2018.
objavljeno
10.1016/j.nbt.2018.05.1082
Podaci o matičnoj publikaciji
European congress of Biotechnology 2018
Lescai, Francesco ; Rudelsheim, Patric
Elsevier
Podaci o skupu
European Congress on Biotechnology
poster
01.07.2018-04.07.2018
Ženeva, Švicarska
Povezanost rada
Kemijsko inženjerstvo, Biotehnologija