Use of an integrated biomarker-based strategy to evaluate physiological stress responses induced by environmental concentrations of caffeine in the Mediterranean mussel Mytilus galloprovincialis (CROSBI ID 663706)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Ćosić, Dorotea ; Iljić, Lucija ; Žmarić, Marko ; Pustijanac, Emina ; Kovačić, Ines
engleski
Use of an integrated biomarker-based strategy to evaluate physiological stress responses induced by environmental concentrations of caffeine in the Mediterranean mussel Mytilus galloprovincialis
Caffeine is an emerging contaminant that is widespread in the aquatic systems, especially in the coastal waters of marine environments. Although naturally caffeine has high biodegradation rates, it is still being investigated at concentrations that have no direct impact on the marine organisms ; but its present could be potential of future stress. Standard approaches cannot provide a reliable estimate, which is why its impact is studied using different groups of biomarkers. In this study, the effect of 5, 50 and 500 ng / L caffeine on mussel Mytilus galloprovincialis was investigated during 7 days. The concentration of caffeine in mussel tissue was measured in addition to lysosomal parameters (lysosomal membrane stability in haemocytes, levels of unsaturated neutral lipids from the digestive gland, lipofuscin levels, lysosomal volume ratio and cytoplasm) ; lipid peroxidation (oxidative stress), acetylcholinesterase activity and primary DNA damage level. Integration of the measured parameters revealed a low level of stress at lower caffeine concentrations, while the highest caffeine concentration caused a decrease in the stability of the lysosomal membrane, the accumulation of neutral lipids and an increased level of antioxidant enzymes. An integrated biomarker-based strategy provides a complete response of the degree of stress on marine invertebrates.
caffeine ; integrated biomarker-based strategy ; mussel
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Podaci o prilogu
9-9.
2018.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts
Habuda-Stanić, Mirna
Osijek: Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku
978-953-7005-56-6
Podaci o skupu
1. međunarodna studentska GREEN konferencija = 1st International Students’ GREEN Conference (ISC GREEN 2018)
predavanje
17.05.2018-18.05.2018
Osijek, Hrvatska