engleski

Metal bioaccumulation and biomarker responses in the liver of Prussian carp (Carassius gibelio Bloch, 1782) from the Croatian river Ilova

The study aim was to determine whether the wastewater input from a petrochemical factory into the Ilova River has caused contamination of the river water that could affect the aquatic organisms and the environment. The concentrations of multiple trace and macro elements in the river water and in the liver of Prussian carp (Carassius gibelio) were measured and compared between two sites: the reference site Ilova village, upstream of the wastewater input, and the potentially contaminated site Trebež village, downstream of the input. Stress caused by potential trace and macro element bioaccumulation in fish liver was tested by measuring biomarkers (total cytosolic proteins, metallothionein, glutathione and catalase). The concentrations of dissolved Na, Cs, Cd and Rb in the river-water downstream of the wastewater inflow were generally slightly higher compared to the upstream samples, whereas Mn had higher concentration upstream of wastewater inflow. The majority of total hepatic concentrations of elements showed comparable bioaccumulation levels in the liver at both sites. Only Na, Rb, and Cs had higher concentrations at Trebež village, which was probably a direct consequence of higher exposure in the river-water. Cytosolic proportions of many elements in Prussian carp liver were very high, and for highly toxic metals Ag, Cd and Cs they were approximately 100%, indicating their high metabolic availability and toxic potential. Biomarker levels did not differ between two sampling sites, indicating that the effects of slightly increased exposure to several elements in the river-water and their bioaccumulation in the liver downstream of petrochemical factory were still not observable in the fish.

metals, bioaccumulation, Prussian carp, liver, biomarkers, Ilova River

nije evidentirano