engleski

Principles of chromatography ; tips and tricks in LC analysis

High-performance liquid chromatography (HPLC) emerged as a powerful, versatile and sensitive technique utilized for conducting separation, identification, and quantification. According to that, the focus of the presentation will be on principles of chromatography as one of the leader techniques in modern analytical chemistry. Particular attention will be on hints and tips with troubleshooting problems related with mobile phases, columns, detectors, etc. Therefore, mainly asked questions related with troubleshooting will be discussed. For instance: how to choose the appropriate column and mobile phase, what are the usual mobile phase problems and how to prevent it, how long to equilibrate the column, what are the benefits of normal phase chromatography and when to apply it, troubleshooting with chromatographic baseline, pressure and peak retention times, how to avoid peak fronting and tailing or the split peaks, why is capacity factor one of the most essential parameters, what to do when there is a problem with analysis of polar compounds on C18 column, what is hydrophobic collapse, etc.?

chromatography, HPLC, method development, analysis of polar compounds

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