engleski

Origin and Genetic Diversity of Croatian Common Bean Landraces

Most of the common bean (Phaseolus vulagris L.) production in Croatia is based on local landraces, grown by small-scale farmers in low input production systems. The landraces are commonly known by their traditional names based primarily on seed coat pattern and colour. In order to assess the genetic diversity of Croatian common bean landraces, 174 accessions were analysed using a DNA marker for phaseolin type, microsatellite markers and a DArTseq method of SNP genotyping. The information on phaseolin type and the results of the distance- and model-based analysis of population structure coupled with the morphological characterization of the accessions enabled the thorough classification of the accessions, that was, for the most part, congruent. Out of 174 accessions, 135 were assigned to 14 newly formed morpho-genetic groups originating from three distinct domestication events: (1) Mesoamerican, (2) Andean - indeterminate type, and (3) Andean - determinate type. The rest of accessions could represent putative hybrids among morpho-genetic groups including the hybrids among the gene pools. This study would be a first step into a genome-wide association mapping using a panel of Croatian common bean landraces. In conjunction with a detailed data on bioactive nutrient content, a whole genome association mapping analysis will be carried out to identify quantitative trait loci (QTLs) related to bioactive nutrient content.

Phaseolus vulgaris L. ; landraces ; genetic diversity ; DArTseq ; gene pools

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