engleski

Inhibition of satellite RNA associated cucumber mosaic virus infection by essential oil of Micromeria croatica (Pers.) Schott

The essential oil was isolated from Micromeria croatica (Pers.) Schott, an endemic plant species of the Dinarides mountain range. The plant material was collected at localities on Mt. Velebit (localities Bojinac, Bačić kuk and Stupačinovo) in Croatia. GC and GC-MS analysis identified β-caryophyllene and caryophyllene oxide as the main oil components. Essential oil, β-caryophyllene and caryophyllene oxide, were tested for antiphytoviral activity on local and systemic host plants infected with satellite RNA associated cucumber mosaic virus (CMVsat). Simultaneous inoculation of CMVsat with essential oil or with the dominant components of oil, and the treatment of plants with essential oil or with the dominant components of oil prior to virus inoculation, resulted in a reduction in the number of lesions on leaves of the local host plants. Regardless of whether oil or oil compounds were inoculated simultaneously with the virus, or applied prior to virus inoculation, the virus concentration was reduced in the systemically infected host plants. The results showed a correlation of the antiviral activity of the essential oil with the main oil components: activity of the oil from Bojinac positively correlated with β-caryophyllene, while activity of the oils from Bačić kuk and Stupačinovo correlated with caryophyllene oxide. Gene expression assay showed that treatment with essential oil changed the level of alternative oxidase (Aox) gene expression in infected Arabidopsis plants. The involvement of Aox in the defence pathways against pathogens indicates a connection between the essential oil treatment, Aox gene expression and the development of viral infection.

Micromeria croatica ; essential oil ; antiphytoviral activity ; β-caryophyllene ; caryophyllene oxide

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