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Pregled bibliografske jedinice broj: 942658

Activity of fosfomycin against nosocomial multiresistant bacteria pathogens from Croatia: a multicentric study


Bielen, Luka; Likić, Robert; Erdeljić, Viktorija; Mareković, Ivana; Firis, Nataša; Grgić-Medić, Marijana; Godan, Ana; Tomić, Ivan; Hunjak, Blaženka; Markotić, Alemka et al.
Activity of fosfomycin against nosocomial multiresistant bacteria pathogens from Croatia: a multicentric study // Croatian medical journal, 30 (2018), 59; 56-64 (međunarodna recenzija, članak, znanstveni)


Naslov
Activity of fosfomycin against nosocomial multiresistant bacteria pathogens from Croatia: a multicentric study

Autori
Bielen, Luka ; Likić, Robert ; Erdeljić, Viktorija ; Mareković, Ivana ; Firis, Nataša ; Grgić-Medić, Marijana ; Godan, Ana ; Tomić, Ivan ; Hunjak, Blaženka ; Markotić, Alemka ; Bejuk, Danijela ; Tičić, Vlatka ; Balzar, Silvana ; Bedenić, Branka

Izvornik
Croatian medical journal (0353-9504) 30 (2018), 59; 56-64

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fosfomycin, extended-spectrum beta-lactamases, carbapenemases, resistance
(Fosfomycin, extended-spectrum beta-lactamases, carbapenema)

Sažetak
Abstract Aim: To determine in vitro susceptibility of multiresistant bacterial isolates to fosfomycin. Methods: In this prospective in vitro study a total of 288 consecutively collected multiresistant bacterial isolates (local non- random sample, level of evidence 3) from seven medical centres in Croatia from February 2014 until October 2016 were tested for susceptibility to fosfomycin and other antibiotics according to Clinical and Laboratory Standards Institute methodology. Susceptibility to fosfomycin was determined by agar dilution method, while disc diffusion was performed for in vitro testing of other antibiotics. Polymerase chain reaction and sequencing was performed for majority of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae (K. pneumoniae) and carbapenem resistant isolates. Results: Majority of 288 multiresistant bacterial isolates (82.6%) were susceptible to fosfomycin. The 236 tested multiresistant Gram negative isolates showed excellent susceptibility to fosfomycin. Susceptibility rates were: Escherichia coli ESBL 97%, K. pneumoniae ESBL 80%, Enterobacter species. 85.7%, Citrobacter freundii 100%, Proteus mirabilis 93%, Pseudomonas aeruginosa 60%. Of the 52 multiresistant Gram positive isolates, fosfomycin demonstrated excellent activity against methicillin-resistant Staphylococcus aureus (94.4%) with low activity against vancomycin resistant enterococcus (31%). Polymerase chain reaction analysis of 36/50 ESBL producing K. pneumoniae isolates showed that majority of isolates had CTX-M-15 beta lactamase (27/36) with ISEcp insertion sequence. All carbapenem resistant Enterobacter and Citrobacter isolates had blaVIM-1 metallo- beta-lactamase gene. Conclusion: With the best in vitro activity among tested antibiotics, fosfomycin, an old antibiotic that became widely available in Croatia, following the country's ascension into the European Union, could be an effective antibiotic for treatment of infections caused by multiresistant Gram negative and Gram positive bacterial strains in the hospital setting.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1080114-0015 - Mehanizmi rezistencije na antibiotike u Gram-negativnih bakterija (Branka Bedenić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE