engleski

The effect of training and the Alka competition on oxidative stress and metabolic parameters in two horse breeds

The aim of this study was to investigate the effects of training and the Alka competition upon the activity/ concentrations of antioxidative and muscle enzymes and metabolic parameters, as well as their correlations, in the serum of Thoroughbred (TH) and Croatian Warmblood (CWH) horses. The experiment was carried out on nine THs and seven CWHs. Blood samples were collected 30, 21 and 14 days before the Alka competition, immediately after the competition, and 7 days after. Serum concentrations of malondialdehyde (MDA), the activity of glutathione peroxidase (GSH-Px), total superoxide dismutase (SOD), paraoxonase 1 (PON 1), creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined, as well as glucose, triglyceride, nonesterifed fatty acids (NEFA), total cholesterol and high density lipoprotein cholesterol (HDL-C) concentrations. Serum MDA concentrations in both breeds were signifcantly higher 30 days before the Alka than immediately after the race. Serum activity of SOD in TH was signifcantly higher 21 days before the Alka compared to the values after 7 days of rest. Serum NEFA concentration in TH was also signifcantly higher 30 days before the Alka and immediately after the race, compared to concentrations 7 days after the race. Triglyceride concentrations in TH were signifcantly higher after 7 days of rest compared to 30 and 14 days before the race. The PON1/HDL-C ratio was signifcantly higher in CWH than in TH serum 21 days before the Alka. MDA values were signifcantly lower in CWH than in TH 14 days before the Alka. Signifcantly higher AST and CK serum activity was determined in CWH than in TH 30 days before Alka. Triglyceride concentrations 21 days before the competition were signifcantly higher in CWH compared to values in TH. The results indicate the oxidative stability of horse serum during training and competition, breed differences during training and competition, and a shift in fat metabolism due to the elevated energy needs.

horses ; exercise ; antioxidative enzymes ; oxidative stress ; metabolic biomarkers

nije evidentirano