engleski

EFFECT OF FURFURAL ON GROWTH AND ACTIVITY OF LACTIC ACID BACTERIA

Lactic acid is mainly produced by lactic acid bacteria (LAB ; Lactobacillus sp., Streptococcus sp., Pediococcus sp.) and molds (e. g. Rhizopus oryzae, Rhizopus arrhizus)[1]. This bulk chemical can be produced from first and second generation raw materials [2]. First generation raw materials are feedstocks that contain sugar and starch and are widely used in industrial-scale lactic acid production. Second generation raw materials are ligncellulosic raw materials that have to be pretreated prior to fermentation [3]. During pretreatment variety of toxic compounds like furfural, hydroxymethylfurfural, levulinic and acetic acid and different lignin derivatives are beeing formed [4]. Obtained hydrolysates are usually treated by different expensive and long-lasting methods that can be used for detoxification procedures. Therefore, tolerance of working microorganism to above mentioned toxic compounds could be an asset in lactic acid production process [5]. Furans can be used as good predictor of the inhibition effect of other toxic substances present in lignocellulosic hydrolysate [2] and furfural was identified as main inhibiting compound obtained after dilute acid pretratment of lignocellulose [6]. Since there is limited amount of data available on toxic effect of inhibitors formed during pretreatment of lignocellulose [6], in this work the influence of different concentrations of furfural (from 100 to 5000 mg L-1) on growth and activity of lactic acid bacterium Lactobacillus amylovorus DSM 20531T in De Man-Rogosa-Sharpe (MRS) medium was investigated.

influence of furfural, lactic acid bacteria, lactic acid production

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