Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 941780

Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks


Šamanić, I.; Cvitanić, R.; Simunić, Juraj; Puizina, J.
Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks // Plant Biology, 18 (2016), 4; 681-694 doi:10.1111/plb.12453 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 941780 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks

Autori
Šamanić, I. ; Cvitanić, R. ; Simunić, Juraj ; Puizina, J.

Izvornik
Plant Biology (1435-8603) 18 (2016), 4; 681-694

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
atmre1 mutants ; ataxia telangiectasia-mutated ; CYCB1 ; 1::GUS ; DNA damage response ; genome instability

Sažetak
Given the fundamental role of MRE11 in many aspects of DNA metabolism and signalling in eukaryotes, we analysed the impact of several MRE11 mutations on DNA damage response (DDR) and DNA repair in Arabidopsis thaliana. Three different atmre11 and an atatm-2 mutant lines, together with the wild type (WT), were compared using a new Arabidopsis genotoxic assay for insitu evaluation of genome integrity and DNA damage repair efficiency after double strand break (DSB) induction. The results showed that, despite the phenotypic differences and different lengths of the putative truncated AtMRE11 proteins, all three atmre11 and the atatm-2 mutant lines exhibited common hypersensitivity to bleomycin treatment, where they only slightly reduced mitotic activity, indicating a G2/M checkpoint abrogation. In contrast to the WT, which reduced the frequency of chromosomal aberrations throughout the recovery period after treatment, none of the three atmre11 and atatm-2 mutants recovered. Moreover, atmre11-3 mutants, similarly to atatm-2 mutants, failed to transcriptionally induce several DDR genes and had altered expression of the CYCB1 ; 1::GUS protein. Nevertheless, numerous chromosomal fusions in the atmre11 mutants, observed after DNA damage induction, suggest intensive DNA repair activity. These results indicate that functional and full-length AtMRE11 is essential for activation of the cell cycle arrest, transcriptional regulation and DNA repair upon induction of DSB.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
177-11911196

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Split

Profili:

Avatar Url Jasna Puizina (autor)

Avatar Url Juraj Simunić (autor)

Avatar Url Ivica Šamanić (autor)

Citiraj ovu publikaciju

Šamanić, I.; Cvitanić, R.; Simunić, Juraj; Puizina, J.
Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks // Plant Biology, 18 (2016), 4; 681-694 doi:10.1111/plb.12453 (međunarodna recenzija, članak, znanstveni)
Šamanić, I., Cvitanić, R., Simunić, J. & Puizina, J. (2016) Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks. Plant Biology, 18 (4), 681-694 doi:10.1111/plb.12453.
@article{article, year = {2016}, pages = {681-694}, DOI = {10.1111/plb.12453}, keywords = {atmre1 mutants, ataxia telangiectasia-mutated, CYCB1, 1::GUS, DNA damage response, genome instability}, journal = {Plant Biology}, doi = {10.1111/plb.12453}, volume = {18}, number = {4}, issn = {1435-8603}, title = {Arabidopsis thalianaMRE11 is essential for activation of cell cycle arrest, transcriptional regulation and DNA repair upon the induction of double-stranded DNA breaks}, keyword = {atmre1 mutants, ataxia telangiectasia-mutated, CYCB1, 1::GUS, DNA damage response, genome instability} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati