Napredna pretraga

Pregled bibliografske jedinice broj: 941418

Initial characterization of dog serum phosphoproteome


Galan, Asier; Horvatić, Anita; Guillemin, Nicolas; Kuleš, Josipa; Bilić Petra; Mrljak, Vladimir
Initial characterization of dog serum phosphoproteome // Proteomics Methods Forum 2017
Oxford, Ujedinjeno Kraljevstvo, 2017. (predavanje, podatak o recenziji nije dostupan, neobjavljeni rad, znanstveni)


Naslov
Initial characterization of dog serum phosphoproteome

Autori
Galan, Asier ; Horvatić, Anita ; Guillemin, Nicolas ; Kuleš, Josipa ; Bilić Petra ; Mrljak, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Proteomics Methods Forum 2017

Mjesto i datum
Oxford, Ujedinjeno Kraljevstvo, 22-23.06.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Dog, serum, phosphoproteome, babesiosis

Sažetak
Phosphorylation is the most commonly studied protein post-translational modification (PTM) in biological systems due to its importance in controlling division, survival, cell growth etc. Nevertheless, there is little information on general phosphoproteomic characterization and description of the content of circulating phosphoproteins. We have performed a gel-free analysis of dog serum samples using LC-MS/MS supported by phosphopeptide enrichment. We compared serum from healthy dogs and dogs affected by B. canis-caused babesiosis. After digestion, phosphopeptides present in samples were enriched using TiO2 magnetic beads. Our approach was both qualitative and quantitative using TMT-labeled serum pool samples in combination with LC-MS/MS.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-4135 - Proteomski pristup otkrivanju biomarkera ranog oštećenja bubrega i srca u pasa (Vladimir Mrljak, )

Ustanove
Veterinarski fakultet, Zagreb