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EXTRACTION OF PYRETHRINS FROM DALMATIAN PYRETHRUM (TANACETUM CINERARIIFOLIUM /Trevir./Sch. Bip.) BY MATRIX-SOLID PHASE DISPERSION

Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir. /Sch. Bip.) is a plant species of the Asteraceae family, restricted to the eastern coast of the Adriatic Sea. It is a perennial plant species known as the source of natural insecticide pyrethrin. The term pyrethrin refers to the six insecticide active components: pyrethrin I and II, cinerin I and II, jasmolin I and II. Insecticidal potential of Dalmatian pyrethrum is measured by the content and composition of pyrethrins which are mainly concentrated in the flower heads. In order to obtain deeper knowledge of its insecticidal potential, content and composition of pyrethrins was assessed by matrix-solid phase extraction (MSPD). The preliminary experiments were carried out to optimize the main parameters affecting MSPD, such as the type of sorbent and the solvent polarity. Results showed that pyrethrins I and pyrethrins II were successfully extracted by florisil as a sorbent using 10 mL of acetone : ethyl-acetate = 1:1 (v/v) as an elution solvent. All experiments were performed using 1:2 ratio of flower heads and sorbent. Extraction efficiency was determined by high performance liquid chromatography with diode array detector (HPLC- DAD). Separation and quantification of the selected components were carried out under gradient elution of mobile phase (0.1% formic acid in water and 0.1% formic acid in acetonitrile) based on Luna C18(2) column, 250 mm x 4.6 mm, particle size 5 μm (Phenomenex).

pyrethrins, matrix-solid phase dispersion, high performance liquid chromatography

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