engleski

Genotoxicity evaluation of chlopyrifos, imidacloprid and α-cypermethrin in low concentrations on human peripheral blood lymphocytes by cytokinesis-block microncleus assay

The use of insecticides is increasing in modern society, but their toxic effects in low concentrations relevant for real scenario exposure have not received large attention. We have treated whole peripheral blood in vitro individually with three active insecticidal substances chlorpyrifos (a conventionally used organophosphate), imidacloprid (a novel neonicotinoid) and α-cypermethrin (a pyrethroid). Applied concentrations were estimate equivalents of acceptable daily intake (ADI), residual exposure level (REL), occupational exposure level (OEL) and a fourth common concentration (3 μg/mL). After treatment according to cytokinesis-block micronucleus assay, binucleated lymphocytes were analyzed for presence of micronuclei (MN), nuclear buds (NB) and nucleoplasmic bridges (NPB), and ratios of mono-, bi-, tri- and tetranuclear cells were calculated for estimation of cytokinesis-block proliferation index (CBPI). The results have shown that none of the tested substances exhibited significant induction of any markers of secondary DNA damage or effect on cell proliferation rates. We conclude that chlorpyrifos, imidacloprid and α-cypermethrin induce no significant secondary DNA damage nor affect proliferation across the tested concentrations.

chlorpyrifos, imidacloprid, α-cypermethrin, micronucleus assay, genotoxicity

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