hrvatski

Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds

Chemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by LC-MS/MS analysis. The main requirements in regard to the good derivatization reagent are fast, selective and quantitative reaction with carbonyl compounds, and efficient fragmentation upon collision-induced dissociation (CID). We have prepared glutamic acid-related hydrazine reagent, and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The CID spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure-specific fragment ions were selected for the development of sensitive multiple reaction monitoring (MRM) method.

carbonyl compounds ; derivatization ; MS/MS analysis ; hydrazine reagent ; hydrazones

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