engleski

Diclazuril residues in liver of broiler chickens following drug administration

A non-ionophoric synthetic coccidiostatic diclazuril is commonly used for the prevention and treatment of coccidiosis on poultry farms. According to Commission Regulation (EU) 2015/46 diclazuril is authorised in complete feed in chickens reared for laying at concentration of 0.8-1.2 mg kg-1. In this study, diclazuril residues were measured in the liver of broilers (n=35) treated with feed medicated with diclazuril at concentrations of 5 mg kg-1 for 21 days, i.e. a dose about 4 times the maximum authorised level. Control group received feed free of coccidiostats. The broilers were killed and liver tissue was collected over a period of 17 days after the end of administration. Diclazuril concentrations were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionisation. Limit of quantification of the method was 12.5 ug kg-1 and recovery 99.1%. The maximum concentrations of 673.7 ug kg-1 were measured on the first post-treatment day. On the fifth day of post-treatment concentrations was 478.9 ug kg-1. After, concentrations gradually dropped from the seventh to fifteenth day after administration from 427.0 to 138.9 ug kg-1. On the seventeen days after the end of treatment, last day of measuring, diclazuril levels were still above the limit of quantification (55.5 ug kg-1).

diclazuril ; liver ; broiler chicken ; residues ; LC-MS/MS

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