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Analysis of MSH2 Loss of Heterozygosity, Expression, and IVS10+12G>A Polymorphism in Sporadic Colon Cancer (CROSBI ID 251638)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Čačev, Tamara ; Zapletal, Emilija ; Musani, Vesna ; Rako, Ivana ; Lončar, Božo ; Aralica, Gorana ; Kapitanović, Sanja Analysis of MSH2 Loss of Heterozygosity, Expression, and IVS10+12G>A Polymorphism in Sporadic Colon Cancer // Anticancer Research, 38 (2018), 5; 2841-2848. doi: 10.21873/anticanres.12529

Podaci o odgovornosti

Čačev, Tamara ; Zapletal, Emilija ; Musani, Vesna ; Rako, Ivana ; Lončar, Božo ; Aralica, Gorana ; Kapitanović, Sanja

engleski

Analysis of MSH2 Loss of Heterozygosity, Expression, and IVS10+12G>A Polymorphism in Sporadic Colon Cancer

Background: mutS homolog 2 (MSH2) deficiency may be involved in the development of microsatellite instability found in certain sporadic colorectal tumors. In addition to mutations or loss of heterozygosity resulting in complete loss of MSH2 function, polymorphisms affecting MSH2 expression have been also identified. Therefore, the aim of this study was to examine MSH2 status in sporadic colon cancer. Materials and Methods: MSH2 status was examined at the DNA, RNA and protein levels through loss of heterozygosity (LOH) analysis, quantitative real-time PCR and immunohistochemistry. MSH2 IVS10+12A>G polymorphism was examined by real-time single nucleotide polymorphism genotyping. Results: MSH2 LOH was more frequent in tumors larger than 5 cm (p=0.032), mRNA expression was also significantly lower and the same expression pattern was present in the corresponding normal mucosa of the same patient (p=0.013 and p=0.008, respectively). No association was found between IVS10+12A>G polymorphism and susceptibility to sporadic colon cancer. Conclusion: Altered MSH2 expression detected in sporadic colon tumors pointing to its role in colorectal tumorigenesis without a hereditary component.

MSH2 gene ; LOH ; mRNA expression ; immunohistochemistry ; IVS10+12G>A ; sporadic colon cancer

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

38 (5)

2018.

2841-2848

objavljeno

0250-7005

1791-7530

10.21873/anticanres.12529

Trošak objave rada u otvorenom pristupu

Povezanost rada

Temeljne medicinske znanosti

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