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Pregled bibliografske jedinice broj: 939689

Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability


Klemm, Anna H.; Bosilj, Agneza; Glunčić, Matko; Pavin, Nenad; Tolić, Iva Marija
Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability // Molecular biology of the cell, 29 (2018), 11; 1332-1345 doi:10.1091/mbc.E17-11-0667 (međunarodna recenzija, članak, znanstveni)


Naslov
Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability

Autori
Klemm, Anna H. ; Bosilj, Agneza ; Glunčić, Matko ; Pavin, Nenad ; Tolić, Iva Marija

Izvornik
Molecular biology of the cell (1059-1524) 29 (2018), 11; 1332-1345

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mitotic spindle ; kinetochore microtubules ; kinesin-8 motors ; microtubule dynamic instability ; kinetochore oscillations ; metaphase model

Sažetak
During metaphase, sister chromatids are connected to microtubules extending from the opposite spindle poles via kinetochores, protein complexes on the chromosome. Kinetochores congress to the equatorial plane of the spindle and oscillate around it, with kinesin-8 motors restricting these movements. Yet, the physical mechanism underlying kinetochore movements is unclear. We show that kinetochore movements in the fission yeast Schizosaccharomyces pombe are regulated by kinesin-8-promoted microtubule catastrophe, force-induced rescue and microtubule dynamic instability. A candidate screen showed that among the selected motors only kinesin-8 motors Klp5/Klp6 are required for kinetochore centering. Kinesin-8 accumulates at the end of microtubules, where it promotes catastrophe. Laser ablation of the spindle resulted in kinetochore movement towards the intact spindle pole in wild-type and klp5Δ cells, suggesting that kinetochore movement is driven by pulling forces. Our theoretical model with Langevin description of microtubule dynamic instability shows that kinesin-8 motors are required for kinetochore centering, whereas sensitivity of rescue to force is necessary for the generation of oscillations. We found that irregular kinetochore movements occur for a broader range of parameters than regular oscillations. Thus, our work provides an explanation for how regulation of microtubule dynamic instability contributes to kinetochore congression and the accompanying movements around the spindle center.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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