High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei (CROSBI ID 251380)
Prilog u časopisu | kratko priopćenje | međunarodna recenzija
Podaci o odgovornosti
Girault, Guillaume ; Wattiau, Pierre ; Saqib, Muhammad Arif Nadeem ; Martin B. ; Vorimore, Fabien ; Singha, Harisankar ; Engelsma, Marc Y. ; Roest, Hendrik Ido Jan ; Spicic, Silvio. ; Grunow Roland ; Vicari Nadia ; De Keersmaecker, Sigrid C.J. ; Roosens, Nancy Hélène C.J. ; Fabbi, Massimo ; Tripathi, Bhupendra Naryan ; Zientara, Steṕhan T. ; Madani, Nora ; Laroucau, Karine
engleski
High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub- branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.
Burkholderia mallei ; Genotyping ; HRM
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Podaci o izdanju
63
2018.
1-4
objavljeno
1567-1348
1567-1348
10.1016/j.meegid.2018.05.004
