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Pregled bibliografske jedinice broj: 938920

High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei


Girault, Guillaume; Wattiau, Pierre; Saqib, Muhammad Arif Nadeem; Martin B.; Vorimore, Fabien; Singha, Harisankar; Engelsma, Marc Y.; Roest, Hendrik Ido Jan; Spicic, Silvio.; Grunow Roland et al.
High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei // Infection, Genetics and Evolution, 63 (2018), 1-4 doi:10.1016/j.meegid.2018.05.004 (međunarodna recenzija, kratko priopcenje, znanstveni)


Naslov
High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei

Autori
Girault, Guillaume ; Wattiau, Pierre ; Saqib, Muhammad Arif Nadeem ; Martin B. ; Vorimore, Fabien ; Singha, Harisankar ; Engelsma, Marc Y. ; Roest, Hendrik Ido Jan ; Spicic, Silvio. ; Grunow Roland ; Vicari Nadia ; De Keersmaecker, Sigrid C.J. ; Roosens, Nancy Hélène C.J. ; Fabbi, Massimo ; Tripathi, Bhupendra Naryan ; Zientara, Steṕhan T. ; Madani, Nora ; Laroucau, Karine

Izvornik
Infection, Genetics and Evolution (1567-1348) 63 (2018); 1-4

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
Burkholderia mallei ; Genotyping ; HRM

Sažetak
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub- branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.

Izvorni jezik
Engleski



POVEZANOST RADA


Ustanove
Hrvatski veterinarski institut, Zagreb

Autor s matičnim brojem:
Silvio Špičić, (263513)

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