Role of ST3GAL5 polymorphism (rs72842068) in cervical cancer development (CROSBI ID 661878)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Danolic, Damir ; Heffer, Marija ; Wagner, Jasenka ; Škrlec, Ivana ; Alvir, Ilija ; Mamic, Ivica ; Susnjar, Lucija ; Banovic, Marija ; Puljiz, Mario
engleski
Role of ST3GAL5 polymorphism (rs72842068) in cervical cancer development
Aims: To determine whether polymorphisms in the ST3GAL5 gene represents risk factor for cervical cancer. Method: In total, 164 women participated in our study. Peripheral blood samples were obtained from 81 cervical cancer patients and 83 healthy controls at University Hospital for Tumors, Zagreb, Croatia. The diagnosis of cervical cancer was confirmed by histological examinations of tissues from biopsies or resected specimens. The controls were represented by age-matched females with a normal Pap smear and no previous history of cervical dysplasia or malignant disease. Informed written consent was obtained from all participants. A 3 mL sample of venous blood was collected from each subject into a test tube containing EDTA as anticoagulant. Genomic DNA was extracted by QIAamp DNA Blood Mini Kit (Qiagen Company) according to the manufacturer’s protocol. The ST3GAL5 (rs72842068) single nucleotide polymorphism was genotyped with TaqMan allele- specific PCR amplification technology on an Applied Biosystems 7500/7500 Real-Time PCR System. Results: There was no significant difference between the two groups in the frequency distributions of age (P = 0.786) suggesting that matching of subjects based on this variable was adequate. We detected only one genotype for the ST3GAL5 (rs72842068) polymorphism. All genotyped subjects carried the homozygous wild-type genotype AA, while heterozygous genotype AG and homozygous genotype GG were not detected. Conclusion: The present study did not show association of the ST3GAL5 polymorphism (rs72842068) with cervical cancer, but it opened future research direction. In our opinion, the relationship between ganglioside biosynthesis and cervical cancer is far from being elucidated.
cervical cancer ; polymorphism ; ST3GAL5
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nije evidentirano
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nije evidentirano
nije evidentirano
Podaci o prilogu
728-728.
2017.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
International journal of gynecological cancer
1048-891X
1525-1438
Podaci o skupu
20th International Meeting of the European Society of Gynaecological Oncology
poster
04.11.2017-07.11.2017
Beč, Austrija
Povezanost rada
Glazbena umjetnost, Grafička tehnologija