engleski

Antioxidative response challenged by excess cadmium and water limitation in maize

Cadmium exposure and water deficit are among the most powerful abiotic stress factors affecting crop growth and productivity. Present study was conducted to evaluate the oxidative damage and the antioxidative response management in the leaves of two maize inbred lines (A: B84, B: Os6-2) and their hybrid (AxB: B84xOs6-2) subjected to excess Cd and or no water limitation in the greenhouse experiment during flowering phase. In well- watered conditions, the increase of lipid peroxidation level (LP) in A and AxB affected by Cd indicates its toxicity, while in B higher amount of accumulated proline could be the reason of maintained LP. In A increased activities of guaiacol peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) were shown while in B POD and APX were increased. Water limitation caused decrease in RWC in A, B and AxB. Higher decrease of RWC was observed in combination of stress factors in A and B but not in AxB. The increase of LP and proline content as well as higher activity of POD and APX was observed in A. In B only POD and APX were increased. Increased APX and higher proline accumulation in AxB might be the reason of decreased LP and unchanged POD. Inhibition of CAT activity was observed in A, B and AxB. Obtained results showed different strategies of antioxidative mechanisms in maize inbred lines and their hybrid subjected to investigated stress factors. It seems that hybrid is more tolerant than its parental lines probably due to heterosis and dilution effect.

antioxidant enzymes, cadmium toxicity, maize, proline, water deficit

nije evidentirano