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Pregled bibliografske jedinice broj: 938335

Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach


Bilić, Petra; Guillemin, Nicolas; Kovačević, Alan; Beer Ljubić, Blanka; Jović, Ines; Galan, Asier; Eckersall, Peter David; Burchmore, Richard; Mrljak, Vladimir
Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach // Journal of Proteomics, 179 (2018), 110-121 doi:10.1016/j.jprot.2018.03.007 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 938335 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach

Autori
Bilić, Petra ; Guillemin, Nicolas ; Kovačević, Alan ; Beer Ljubić, Blanka ; Jović, Ines ; Galan, Asier ; Eckersall, Peter David ; Burchmore, Richard ; Mrljak, Vladimir

Izvornik
Journal of Proteomics (1874-3919) 179 (2018); 110-121

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Idiopathic dilated cardiomyopathy ; Dog ; Serum ; Label-based proteomics ; Biomarker ; Bioinformatics

Sažetak
Idiopathic dilated cardiomyopathy (iDCM) is a primary myocardial disorder with an unknown aetiology, characterized by reduced contractility and ventricular dilation of the left or both ventricles. Naturally occurring canine iDCM was used herein to identify serum proteomic signature of the disease compared to the healthy state, providing an insight into underlying mechanisms and revealing proteins with biomarker potential. To achieve this, we used high-throughput label-based quantitative LC-MS/MS proteomics approach and bioinformatics analysis of the in silico inferred interactome protein network created from the initial list of differential proteins. To complement the proteomic analysis, serum biochemical parameters and levels of know biomarkers of cardiac function were measured. Several proteins with biomarker potential were identified, such as inter-alpha-trypsin inhibitor heavy chain H4, microfibril- associated glycoprotein 4 and apolipoprotein A- IV, which were validated using an independent method (Western blotting) and showed high specificity and sensitivity according to the receiver operating characteristic curve analysis. Bioinformatics analysis revealed involvement of different pathways in iDCM, such as complement cascade activation, lipoprotein particles dynamics, elastic fibre formation, GPCR signalling and respiratory electron transport chain.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-4135 - Proteomski pristup otkrivanju biomarkera ranog oštećenja bubrega i srca u pasa (BioDog) (Mrljak, Vladimir, HRZZ - 2013-11) ( POIROT)

Ustanove:
Veterinarski fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Bilić, Petra; Guillemin, Nicolas; Kovačević, Alan; Beer Ljubić, Blanka; Jović, Ines; Galan, Asier; Eckersall, Peter David; Burchmore, Richard; Mrljak, Vladimir
Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach // Journal of Proteomics, 179 (2018), 110-121 doi:10.1016/j.jprot.2018.03.007 (međunarodna recenzija, članak, znanstveni)
Bilić, P., Guillemin, N., Kovačević, A., Beer Ljubić, B., Jović, I., Galan, A., Eckersall, P., Burchmore, R. & Mrljak, V. (2018) Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach. Journal of Proteomics, 179, 110-121 doi:10.1016/j.jprot.2018.03.007.
@article{article, author = {Bili\'{c}, Petra and Guillemin, Nicolas and Kova\v{c}evi\'{c}, Alan and Beer Ljubi\'{c}, Blanka and Jovi\'{c}, Ines and Galan, Asier and Eckersall, Peter David and Burchmore, Richard and Mrljak, Vladimir}, year = {2018}, pages = {110-121}, DOI = {10.1016/j.jprot.2018.03.007}, keywords = {Idiopathic dilated cardiomyopathy, Dog, Serum, Label-based proteomics, Biomarker, Bioinformatics}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2018.03.007}, volume = {179}, issn = {1874-3919}, title = {Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach}, keyword = {Idiopathic dilated cardiomyopathy, Dog, Serum, Label-based proteomics, Biomarker, Bioinformatics} }
@article{article, author = {Bili\'{c}, Petra and Guillemin, Nicolas and Kova\v{c}evi\'{c}, Alan and Beer Ljubi\'{c}, Blanka and Jovi\'{c}, Ines and Galan, Asier and Eckersall, Peter David and Burchmore, Richard and Mrljak, Vladimir}, year = {2018}, pages = {110-121}, DOI = {10.1016/j.jprot.2018.03.007}, keywords = {Idiopathic dilated cardiomyopathy, Dog, Serum, Label-based proteomics, Biomarker, Bioinformatics}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2018.03.007}, volume = {179}, issn = {1874-3919}, title = {Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach}, keyword = {Idiopathic dilated cardiomyopathy, Dog, Serum, Label-based proteomics, Biomarker, Bioinformatics} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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