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Pregled bibliografske jedinice broj: 937715

ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS


(EU COST Action CM1403 UPCON) Pem, Barbara; Ilić, Krunoslav; Pavičić, Ivan; Moros, Maria; Jurado, Manuel Ocaña; Becerro Nieto, Ana Isabel; de la Fuente, Jesús Martínez; Vinković Vrček, Ivana
ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS // Book of Abstract of the 2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials / Acosta D.H.C ; Carrero S. G. ; Soriano L. F. (ur.).
Valencia, Spain: EU COST Action CM1403 UPCON, 2018. str. 123-123 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS

Autori
Pem, Barbara ; Ilić, Krunoslav ; Pavičić, Ivan ; Moros, Maria ; Jurado, Manuel Ocaña ; Becerro Nieto, Ana Isabel ; de la Fuente, Jesús Martínez ; Vinković Vrček, Ivana

Kolaboracija
EU COST Action CM1403 UPCON

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstract of the 2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials / Acosta D.H.C ; Carrero S. G. ; Soriano L. F. - Valencia, Spain : EU COST Action CM1403 UPCON, 2018, 123-123

Skup
2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials

Mjesto i datum
Valencia, Spain, 02-06.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
upconverting nanoparticles, safety assesment, viability assays

Sažetak
MOTIVATION Upconverting nanoparticles (UPNPs) have lately emerged as candidates for bio-imaging and bio-sensing applications, 1 making the evaluation of their biocompatibility and safety an important task. Presently, biomedical evaluation of metallic nanoparticles (NPs) is based on in vitro assays.2 Due to their unique physicochemical properties, such as large surface area and reactivity, NPs are known to interfere with assay components and detection systems.3 RESULTS The aim of this study was to investigate the interferences caused by differently functionalized UPNPs with conventional cytotoxicity tests. We performed a number of common in vitro assays that determine metabolic activity (MTT and MTS reduction) and oxidative stress (DCFH-DA oxidation, total glutathione level) to assess cytotoxicity. The results demonstrated applicability of different in vitro assays for safety evaluation of UPNPs. The extent and type of interference depended on the UPNPs concentration and the type of assay. CONCLUSIONS This study demonstrates the need for appropriate adaptations of experimental design when conducting cytotoxicity studies of UPNPs, and highlights different approaches to more accurately assess nanotoxicity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2016-06-2436 - Značaj interakcija metalnih nanočestica sa sumpornim biomolekulama za nano-bio sučelje (Ivana Vinković Vrček, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Citiraj ovu publikaciju

(EU COST Action CM1403 UPCON) Pem, Barbara; Ilić, Krunoslav; Pavičić, Ivan; Moros, Maria; Jurado, Manuel Ocaña; Becerro Nieto, Ana Isabel; de la Fuente, Jesús Martínez; Vinković Vrček, Ivana
ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS // Book of Abstract of the 2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials / Acosta D.H.C ; Carrero S. G. ; Soriano L. F. (ur.).
Valencia, Spain: EU COST Action CM1403 UPCON, 2018. str. 123-123 (poster, međunarodna recenzija, sažetak, znanstveni)
(EU COST Action CM1403 UPCON) (EU COST Action CM1403 UPCON) Pem, B., Ilić, K., Pavičić, I., Moros, M., Jurado, M., Becerro Nieto, A., de la Fuente, J. & Vinković Vrček, I. (2018) ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS. U: Acosta D.H.C, Carrero S. G. & Soriano L. F. (ur.)Book of Abstract of the 2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials.
@article{article, editor = {Acosta D.H.C, Carrero S. G. and Soriano L. F.}, year = {2018}, pages = {123-123}, keywords = {upconverting nanoparticles, safety assesment, viability assays}, title = {ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS}, keyword = {upconverting nanoparticles, safety assesment, viability assays}, publisher = {EU COST Action CM1403 UPCON}, publisherplace = {Valencia, Spain} }