ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS (CROSBI ID 661646)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Pem, Barbara ; Ilić, Krunoslav ; Pavičić, Ivan ; Moros, Maria ; Jurado, Manuel Ocaña ; Becerro Nieto, Ana Isabel ; de la Fuente, Jesús Martínez ; Vinković Vrček, Ivana
engleski
ASSESSING INTERFERENCES OF UPCONVERTING NANOPARTICLES WITH IN VITRO TOXICITY ASSAYS
MOTIVATION Upconverting nanoparticles (UPNPs) have lately emerged as candidates for bio-imaging and bio-sensing applications, 1 making the evaluation of their biocompatibility and safety an important task. Presently, biomedical evaluation of metallic nanoparticles (NPs) is based on in vitro assays.2 Due to their unique physicochemical properties, such as large surface area and reactivity, NPs are known to interfere with assay components and detection systems.3 RESULTS The aim of this study was to investigate the interferences caused by differently functionalized UPNPs with conventional cytotoxicity tests. We performed a number of common in vitro assays that determine metabolic activity (MTT and MTS reduction) and oxidative stress (DCFH-DA oxidation, total glutathione level) to assess cytotoxicity. The results demonstrated applicability of different in vitro assays for safety evaluation of UPNPs. The extent and type of interference depended on the UPNPs concentration and the type of assay. CONCLUSIONS This study demonstrates the need for appropriate adaptations of experimental design when conducting cytotoxicity studies of UPNPs, and highlights different approaches to more accurately assess nanotoxicity.
upconverting nanoparticles, safety assesment, viability assays
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
123-123.
2018.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstract of the 2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials
Acosta D.H.C ; Carrero S. G. ; Soriano L. F.
Valencia: EU COST Action CM1403 UPCON
Podaci o skupu
2nd Conference and Spring School on Properties, Design and Applications of Upconversion Nanomaterials (UPCON 2018)
poster
02.06.2018-02.06.2018
Valencia, Španjolska