engleski

Report from the Short-Term Scientific Mission (STSM) in Rome on joint work for adjusting database and making the Masterfile and performing first statistical analysis, discussion of the results and preparation for writing the scientific paper

The crucial task in deliverables and milestones of the Working Group 1 in the newly formed Cost Action hCOMET- The comet assay as a human biomonitoring tool, was to make files for data collection from the laboratories using alkaline comet assay in human biomonitoring, to contact and send them spreadsheets and other documents where methods and studies would be described in details. Collected spreadsheets from the participating laboratories were used to create a unified database- Masterfile.After the Masterfile, next task would be to discover firstly the technical factors influencing comet assay results and to check their comparability among the laboratories and give guidelines and protocols in order to minimize the technical influence on the comet assay results. For this reason, the Short-Term Scientific Mission (STSMs) has been done after collection of the data. We have collected data on more than 19 000 persons, from 41 laboratories all over the world with 90 methods described and with the results from 108 studies. In one month during STSM, the data were unified in one database and the first statistical analysis were performed with description of the similarities and differences in methods and performance and type of the studies. The results of the first statistical analysis will be represented and we will represent the changed masterfile after adjustment according to the type of the data received. Masterfile includes not only results on DNA damage parameters, but also DNA damage and repair measured with FPG, hOGG1, ENDO III, BER and NER repair, cellular repair assay, information about clinical parameters, exposure, life-style habits: diet, exercise, BMI ; disease, drugs and, if measured, information about chromosomal aberrations, micronucleus or polymorphisms.

comet assay, database

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