engleski

Can measurement of arsenic concentration be comparable in human buccal cells, hair, samples and urine samples and correlate with DNA damage assessment with micronucleus buccal cytome assay?

Although chronic arsenic (As) exposure is associated with toxic effects, both malignant and non- malignant, the dose response for As toxicity is still not clearly determined for human population, and therefore there are still not reliable biomarkers of the exposure and the effect for chronic As exposure that would helpful in assessment of DNA damage and prediction of cancer risk development. Sampling of urine, hair and buccal cells is part of noninvasive methods, but the potential of those three sampling types is still not fully investigated. With no literature data on arsenic measured in buccal cells and its correlation with DNA damage in buccal cells and arsenic concentrations in hair and urine samples, the aim of this study was to evaluate the buccal cells measurement with two other sampling materials in order to give an insight whether these methods can be used together. As and other elements were measured in more than 100 samples of the individuals from the Eastern Croatia chronically exposed to As through ground water, drinking water (As levels above 10 µg) and water used for crops. Liquid chromatography coupled to inductively coupled plasma mass spectrometry-ICPMS was used and the results were compared with the DNA damage assessed in buccal cells. Results demonstrated that As concentrations determined in buccal cells were not in the correlation with those determined in hair and urine samples. The study was financially supported by the Grant of Ministry of Science and Education of the Republic of Croatia and Austrian Federal Ministry of Science, Research and Economy 05/2016 Possible early noninvasive biomarkers of chronic exposure to arsenic

arsenic ; urine ; buccal cells ; hair ; DNA damage

Hrvatsko-austrijski bilateralni projekt 05/2016 dr Milić i prof Gössler