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Pregled bibliografske jedinice broj: 934035

Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers


Mario, Novak; Pavlečić, Mladen; Harutyunyan, Baghish; Goginyan, Vigen; Horvat, Predrag; Šantek, Božidar
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers // Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12 (2017), 3-4; 113-119 (međunarodna recenzija, članak, znanstveni)


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Naslov
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers

Autori
Mario, Novak ; Pavlečić, Mladen ; Harutyunyan, Baghish ; Goginyan, Vigen ; Horvat, Predrag ; Šantek, Božidar

Izvornik
Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam (1847-3423) 12 (2017), 3-4; 113-119

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
purple non-sulphur bacteria ; Rhodobacter capsulatus ; 5-aminolevulinic acid ; optimization of cultivation conditions.

Sažetak
Wild strains of purple non-sulphur bacteria: Rhodospirillum rubrum B-6505, Rhodopseudomonas palustris B-6506, Rhodobacter capsulatus B-6508 and Rhodobacter spheroides B-6509 were studied as 5-ALA (5 aminolevulinic acid) producers. Selected strains were subjected to mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine to obtain a strain with high 5-ALA producing capacity. After mutagenesis 19 stable mutant strains were selected from Rhodobacter capsulatus B-6508 and Rhodobacter sphaeroides B-6509. On the basis of obtained results, mutant strain of Rhodobacter capsulatus B-6508 has shown the highest potential for 5-ALA production. The most favorable conditions for growth and 5-ALA production by mutant strain R. capsulatus B-6508 were observed in media composed of glutamate and malate, light at 2000 Lux, microaerophilic conditions and temperature of 28 °C. In these conditions, the highest 5- ALA concentration (179 mg/L) was detected together with the highest bacterial physiological activity. The prolongation of mutant strain R. capsulatus B-6508 cultivation time after glycine, succinate and levulinic acid addition is related to the reduction of 5- ALA concentrations (e.g. 124.5 mg/L after 48 h and 89.5 mg/L after 72 hours). In the light/aerobic conditions R. capsulatus B-6508 produced only 58.1 mg/L of 5 ALA. Furthermore, in dark conditions even lower biomass and 5-ALA concentrations were observed during R. capsulatus B-6508 cultivation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
EK-H2020-690925 - People for tHe eurOpean bioENergy mIX (Phoenix) (, EK - H2020-MSCA-RISE-2015 )
KK.01.1.1.01.0002 - BioProspecting Jadranskog mora (BioProspecting Jadranskog mora ) (Čož-Rakovac, Rozelinda; Dragović-Uzelac, Verica; Šantek, Božidar; Jokić, Stela; Jerković, Igor; Kraljević Pavelić, Sandra, EK - Strukturni i investicijski fondovi EU u sklopu Operativnog programa “Konkurentnost i kohezija 2014.-2020.” Poziv: Vrhunska istraživanja Znanstvenih cen-tara izvrsnosti KK.01.1.1.01 )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Mario Novak (autor)

Avatar Url Božidar Šantek (autor)

Avatar Url Predrag Horvat (autor)

Avatar Url Mladen Pavlečić (autor)

Citiraj ovu publikaciju

Mario, Novak; Pavlečić, Mladen; Harutyunyan, Baghish; Goginyan, Vigen; Horvat, Predrag; Šantek, Božidar
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers // Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12 (2017), 3-4; 113-119 (međunarodna recenzija, članak, znanstveni)
Mario, N., Pavlečić, M., Harutyunyan, B., Goginyan, V., Horvat, P. & Šantek, B. (2017) Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers. Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12 (3-4), 113-119.
@article{article, year = {2017}, pages = {113-119}, keywords = {purple non-sulphur bacteria, Rhodobacter capsulatus, 5-aminolevulinic acid, optimization of cultivation conditions.}, journal = {Hrvatski \v{c}asopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam}, volume = {12}, number = {3-4}, issn = {1847-3423}, title = {Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers}, keyword = {purple non-sulphur bacteria, Rhodobacter capsulatus, 5-aminolevulinic acid, optimization of cultivation conditions.} }




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