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Pregled bibliografske jedinice broj: 930282

Characterization of a Group I Nme protein of Capsaspora owczarzaki – a close unicellular relative of animals


Ćetković, Helena; Herak Bosnar, Maja; Perina, Dragutin; Mikoč, Andreja; Deželjin, Martina; Belužić, Robert; Bilandžija, Helena; Ruiz-Trillo, Inaki; Harcet, Matija
Characterization of a Group I Nme protein of Capsaspora owczarzaki – a close unicellular relative of animals // Laboratory investigation, 98 (2018), 3; 304-314 doi:10.1038/labinvest.2017.134 (međunarodna recenzija, članak, znanstveni)


Naslov
Characterization of a Group I Nme protein of Capsaspora owczarzaki – a close unicellular relative of animals

Autori
Ćetković, Helena ; Herak Bosnar, Maja ; Perina, Dragutin ; Mikoč, Andreja ; Deželjin, Martina ; Belužić, Robert ; Bilandžija, Helena ; Ruiz-Trillo, Inaki ; Harcet, Matija

Izvornik
Laboratory investigation (0023-6837) 98 (2018), 3; 304-314

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Metastasis suppressor ; Nme1 ; Nm23 ; Capsaspora owczarzaki ; evolution

Sažetak
Nucleoside diphosphate kinases are enzymes present in all domains of life. In animals, they are called Nme or Nm23 proteins, and are divided into group I and II. Human Nme1 was the first protein identified as a metastasis suppressor. Because of its medical importance, it has been extensively studied. In spite of the large research effort, the exact mechanism of metastasis suppression remains unclear. It is unknown which of the biochemical properties or biological functions are responsible for the antimetastatic role of the mammalian Nme1. Furthermore, it is not clear at which point in the evolution of life group I Nme proteins acquired the potential to suppress metastasis, a process that is usually associated with complex animals. In this study we performed a series of tests and assays on a group I Nme protein from filasterean Capsaspora owczarzaki, a close unicellular relative of animals. The aim was to compare the protein to the well-known human Nme1 and Nme2 homologs, as well as with the homolog from a simple animal - sponge (Porifera), in order to see how the proteins changed with the transition to multicellularity, and subsequently in the evolution of complex animals. We found that premetazoan-type protein is highly similar to the homologs from sponge and human, in terms of biochemical characteristics and potential biological functions. Like the human Nme1 and Nme2, it is able to diminish the migratory potential of human cancer cells in culture.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2513 - Uloga gena nm23 u karcinomu pločastog epitela usne šupljine (Maja Herak-Bosnar, )
098-0982913-2874 - Geni i genomi: struktura, funkcija i evolucija (Helena Ćetković, )
HRZZ-IP-2016-06-4021 - Struktura, funkcija i evolucija proteina Nme6/Nm23-H6 (Maja Herak Bosnar, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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