Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1 (CROSBI ID 249398)
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Podaci o odgovornosti
Milić, Dalibor ; Dick, Markus ; Mulnaes, Daniel ; Pfleger, Christopher ; Kinnen, Anna ; Gohlke, Holger ; Groth, Georg
engleski
Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1
Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein–protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 – the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.
ethylene signaling ; ethylene receptor ; ripening inhibition
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