engleski

RNA adenylation during oocyte-to-embryo transition in mice

All animal embryos undergo a transition during which development is handed from maternal to the zygotic control. In mouse, the oocyte-to-embryo transition is a highly coordinated process largely relying on post-transciptional mechanisms because of transcriptional quiescence between the fully-grown oocyte stage and the activation of gene expression in a cleaving zygote. This makes mechanisms of post-transcriptional control of gene expression primary candidates for essential control elements of the maternal to zygotic transition. One of such mechanisms is covalent modification of RNA 3‘ termini by a family of RNA-specific ribonucletidyl transferases, which add A or U ribonucleotides at the 3´ terminus. Covalent modifications at 3‘ termini plays a role in processing of eukaryotic messenger RNA precursors, control of translation, and RNA quality control. Here, we report our analysis of RNA adenylation during oocyte-to-embryo transition in mice where the maternal transcriptome undergoes distinct waves of RNA adenylation and deadenylation.

development ; embryo ; RNA adenylation ; mRNA processing

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