Napredna pretraga

Pregled bibliografske jedinice broj: 929360

Usporedba ekstrakcija čvrstom i tekućinsko- tekućinskom fazom za određivanje metadona u uzorcima seruma i pune krvi plinskom kromatografijom-masenom spektrometrijom


Veršić Bratinčević, Maja; Visković, Tanja; Sutlović, Davorka
Usporedba ekstrakcija čvrstom i tekućinsko- tekućinskom fazom za određivanje metadona u uzorcima seruma i pune krvi plinskom kromatografijom-masenom spektrometrijom // Arhiv za higijenu rada i toksikologiju, 68 (2017), 4; 308-314 (podatak o recenziji nije dostupan, članak, znanstveni)


Naslov
Usporedba ekstrakcija čvrstom i tekućinsko- tekućinskom fazom za određivanje metadona u uzorcima seruma i pune krvi plinskom kromatografijom-masenom spektrometrijom
(Comparison of the solid phase and liquid-liquid extraction methods for methadone determination in human serum and whole blood samples using gas chromatography/mass spectrometry)

Autori
Veršić Bratinčević, Maja ; Visković, Tanja ; Sutlović, Davorka

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 68 (2017), 4; 308-314

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biološki uzorci ; ekstrakcijske tehnike ; GC/MS ; supstitucijska terapija
(GC/MS ; liquid-liquid extraction ; methadone ; serum samples ; solid-phase extraction ; whole blood samples)

Sažetak
The aim of this study was to determine the optimal biological sample and the optimal extraction technique for monitoring methadone concentrations in biological samples. We analysed methadone in serum and whole blood samples using gas chromatography/mass spectrometry (GC/MS). Before analysis, we compared five solid-phase extraction (SPE) and two liquid-liquid extraction (LLE) methods and determined that SPE with Supelco LC-18 in serum yielded the best extraction efficiency. The limit of detection was 10 ng mL-1 and the limit of quantification 25 ng mL-1. Correlation coefficient was over 0.999 for the methadone calibration curve in linear range from 50 to 2000 ng mL-1. Intra and inter-day accuracy and precision of the method was satisfactory. The method was successfully applied for determining serum methadone in patients on maintenance therapy.

Izvorni jezik
Engleski



POVEZANOST RADA


Ustanove
Medicinski fakultet, Split

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE