engleski

Whole‐cell asymmetric reduction of prochiral ketones in natural deep eutectic solvents

Optically active chiral building blocks are widely used as intermediates in the production of drugs, organic chemicals and agricultural chemicals. As the conventional, environmentally hazardous chemical synthesis is expensive and extremely complicated, there was a rising need to introduce environmentally friendly approaches into the preparation of various commercial products. Herein, two tools of green chemistry were used: performing reactions by biocatalysis and use of alternative green solvents – natural deep eutectic solvents (NADES). For that purpose, due to stereoselectivity of the plant and yeast enzymes, two plant callus cultures - sugar beet and cactus, and baker's yeast Saccharomyces cerevisiae were used as biocatalysts for the asymmetric reduction of 3- methylacetophenone, 3, 4-dimethylacetophenone and 2, 4, 6- trimethylacetophenone into corresponding optically active phenyl-ethanol in choline chloride-based NADES. In summary, we successfully developed an eco- friendly enantioselective reduction in cholinium-based NADES. However, the impact of NADES on whole- cell biocatalysis cannot be simply explained by a single interpretation involving a complicated interaction between them.

asymmetric reduction ; natural deep eutectic solvents ; whole-cell biocatalysis

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