Napredna pretraga

Pregled bibliografske jedinice broj: 927092

Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments


Gacesa, Ranko; Baranasic, Damir; Starcevic, Antonio; Diminic, Janko; Korlević, Marino; Najdek-Dragić, Mirjana; Blažina, Maria; Oršolić, Davor; Kolesarić, Domagoj; Long, Paul F. et al.
Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments // Food technology and biotechnology, 56 (2018), 2; 270-277 doi:10.17113/ftb.56.02.18.5393 (međunarodna recenzija, kratko priopcenje, znanstveni)


Naslov
Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Autori
Gacesa, Ranko ; Baranasic, Damir ; Starcevic, Antonio ; Diminic, Janko ; Korlević, Marino ; Najdek-Dragić, Mirjana ; Blažina, Maria ; Oršolić, Davor ; Kolesarić, Domagoj ; Long, Paul F. ; Cullum, John ; Hranueli, Daslav ; Orlić, Sandi ; Zucko, Jurica ;

Izvornik
Food technology and biotechnology (1330-9862) 56 (2018), 2; 270-277

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
Oil pollution ; n-alkane degradation, database

Sažetak
Three metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM-profiles was developed to detect putative genes for hydrocarbon-degrading enzymes. Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA


Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati