Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Pyrene – cyanine conjugates as multipurpose fluorescent probes for non-covalent recognition of ds-DNA, RNA and proteins. (CROSBI ID 248678)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Šmidlehner, Tamara ; Badovinac, Marko ; Piantanida, Ivo Pyrene – cyanine conjugates as multipurpose fluorescent probes for non-covalent recognition of ds-DNA, RNA and proteins. // New journal of chemistry, 42 (2018), 9; 6655-6663. doi: 10.1039/c8nj00055g

Podaci o odgovornosti

Šmidlehner, Tamara ; Badovinac, Marko ; Piantanida, Ivo

engleski

Pyrene – cyanine conjugates as multipurpose fluorescent probes for non-covalent recognition of ds-DNA, RNA and proteins.

Two novel conjugates of pyrene and cyanine were constructed by linking them with the rigid triazole-peptide linker. New probes bind very strongly (with 0.1 µM affinity) to both, ds- DNA(RNA) and proteins (BSA), giving significantly different fluorimetric response: strong pyrene emission change highly selective for protein and “switch-on” of cyanine fluorescence highly selective for DNA(RNA). Moreover, new probes yield induced CD bands only with DNA/RNA, but not with BSA, which allowed independent check of DNA presence in DNA/protein mixtures. Furthermore, probes contain FRET pair of chromophores, whereby FRET is silent in a free molecule solution and activated by binding small molecule to biomacromolecular target. The efficiency of the FRET is to some extent related to the secondary structure of DNA/RNA and only for one of the probes FRET is activated in protein. Two probes show distinctively different induced CD pattern in 400-600 nm range (attributed to a different position of linker attachment on cyanine core), allowing differentiation between various secondary structures of DNA or RNA, showed to be additionally enhanced by combining pyrene and cyanine in one molecule. Due to low cytotoxicity and efficient cellular uptake, probes are good candidates for further biological studies.

DNA/RNA/protein recognition, FRET, pyrene, cyanine

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

42 (9)

2018.

6655-6663

objavljeno

1144-0546

1369-9261

10.1039/c8nj00055g

Povezanost rada

Kemija

Poveznice
Indeksiranost