engleski

ANALYSIS OF INTESTINE MICROBIOME IN THE ALZHEIMER'S DISEASE RAT MODEL

Introduction. Alzheimer's disease (AD) is progressive neurodegenerative disease and leading cause of cognitive and behaviour impairments of industrialized society. Microbiome-gut-brain axis is bidirectional communication between central and enteric nervous system, thus connecting emotional and cognitive centers in brain with peripheral gut function. Aim. Aim of the research is to explore changes in the microbiome that happened due to treatment of the rats with aluminium chloride and D-galactose. Materials and methods. In this research rat model of AD (n=10) was induced by intraperitoneal injection of aluminium chloride (10 mg/kg rat) and D- galactose (60 mg/kg rat) during 28 days. Dilutions were made from rat's colon content and streaked on selective plates for isolation of Lactobacillus and Bifidobacter, activity of bacterial enzymes was analyzed and DNA isolated for sequencing. Sequencing data was analyzed by hierarchical clustering and principal component analysis. Results. Results showed: a) reduced number of probiotic bacteria in AD model compared to control group ; b) increased activity of β- galactosidase, β-glucosidase i β-glucuronidase in AD model compared to control group c) there was no great changes in composition of intestine microbiome, but 46 bacterial species, which make 11, 61% of intestine microbiome of control group, significantly changed. Conclusion. Based on these results it can be concluded that reduced number of Lactobacillus and Bifidobacter, increased enzyme activity of β-galactosidase, β-glucosidase i β- glucuronidase and reduced intestine biomass are connected with inflammation induced in AD rat model.

Alzheimer's disease, aluminium chloride, D-galactose, intestine microbiome, β-galactosidase, β-glucosidase, β-glucuronidase

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