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Pregled bibliografske jedinice broj: 921989

Cigarette smoke induces activation of polymorphonuclear leukocytes


Somborac Bačura, Anita; Popović-Grle, Sanja; Zovko, Vlasta; Žanić-Grubišić, Tihana
Cigarette smoke induces activation of polymorphonuclear leukocytes // Lung, 196 (2018), 1; 27-31 doi:10.1007/s00408-017-0077-3 (međunarodna recenzija, članak, znanstveni)


Naslov
Cigarette smoke induces activation of polymorphonuclear leukocytes

Autori
Somborac Bačura, Anita ; Popović-Grle, Sanja ; Zovko, Vlasta ; Žanić-Grubišić, Tihana

Izvornik
Lung (0341-2040) 196 (2018), 1; 27-31

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chronic obstructive pulmonary disease ; cigarette smoke ; matrix metalloproteinase-9 ; polymorphonuclear leukocytes

Sažetak
Introduction: Cigarette smoking is a major risk factor for chronic obstructive pulmonary disease (COPD). Exposure to cigarette smoke may stimulate inflammatory response and activate polymorphonuclear leukocytes (PMN) thus resulting in secretion of cellular proteases. The aim of our study was to explore the effect of cigarette smoke extract (CSE) on the release of matrix metalloproteinase-9 (MMP-9) from PMN. Methods: The study included 23 patients with stable COPD and 9 healthy controls. PMN were isolated from blood of all participants and exposed to 4 % CSE or basal culture medium (0 % CSE) for 20 h. MMP-9 concentration in PMN culture media was measured using the ELISA method. Results: Exposure of PMN to 4 % CSE did not cause cytotoxic effects, as determined by no changes in lactate dehydrogenase (LDH) activity in PMN culture media when compared to untreated PMN (P=0.689). In basal conditions PMN of COPD patients released significantly more MMP-9 compared with PMN of healthy controls (P=0.016). However, concentration ratio of MMP-9 released from PMN exposed to 4 % CSE or 0 % CSE of each participant was significantly higher for healthy subjects than for COPD patients (P=0.025). Conclusion: Cigarette smoke induces activation of PMN in healthy controls. However, chronically activated PMN in COPD patients could not be further stimulated by in vitro exposure to CSE. Constantly raised amount of MMP-9 released into the tissues may be involved in the degradation of extracellular matrix in the lungs as seen in COPD patients.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
006-0061245-0977 - Molekularni mehanizmi patogeneze kronične opstrukcijske bolesti pluća (Tihana Žanić-Grubišić, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinika za plućne bolesti "Jordanovac"

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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